Recombinant Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13574(B)-30] to SMYD1
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SMYD1 antibody [EPR13574(B)-30]
See all SMYD1 primary antibodies -
Description
Rabbit monoclonal [EPR13574(B)-30] to SMYD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human skeletal muscle and fetal heart lysates; Human heart tissue and skeletal muscle tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13574(B)-30 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 57 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 57 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
Target
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Function
Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis. -
Sequence similarities
Contains 1 MYND-type zinc finger.
Contains 1 SET domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 150572 Human
- Entrez Gene: 12180 Mouse
- Entrez Gene: 297333 Rat
- Omim: 606846 Human
- SwissProt: Q8NB12 Human
- SwissProt: P97443 Mouse
- Unigene: 516176 Human
- Unigene: 234274 Mouse
see all -
Alternative names
- BOP antibody
- CD8 beta opposite antibody
- CD8b opposite antibody
see all
Images
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All lanes : Anti-SMYD1 antibody [EPR13574(B)-30] (ab181372) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human skeletal muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 57 kDa -
Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling SMYD1 with ab181372 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling SMYD1 with ab181372 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal heart lysate or (Lane 2) 1XPBS (negative control) immunoprecipitated using ab181372 at 1/10 dilution with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab181372 has been referenced in 2 publications.
- Sakamoto T et al. The nuclear receptor ERR cooperates with the cardiogenic factor GATA4 to orchestrate cardiomyocyte maturation. Nat Commun 13:1991 (2022). PubMed: 35418170
- Sauls K et al. Initiating Events in Direct Cardiomyocyte Reprogramming. Cell Rep 22:1913-1922 (2018). WB ; Mouse . PubMed: 29444441