Anti-SNAP23 antibody (ab4114)
Key features and details
- Rabbit polyclonal to SNAP23
- Suitable for: IHC-Fr, IP, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SNAP23 antibody
See all SNAP23 primary antibodies -
Description
Rabbit polyclonal to SNAP23 -
Host species
Rabbit -
Specificity
This antibody reacts specifically with human SNAP23. -
Tested applications
Suitable for: IHC-Fr, IP, WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Fusion protein corresponding to Human SNAP23. Immunogen is a SNAP23-GST fusion protein
Database link: O00161 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 17272274
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IP |
Use at an assay dependent concentration. PubMed: 22393054
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WB | (1) |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
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IHC-Fr
Use at an assay dependent concentration. PubMed: 17272274 |
IP
Use at an assay dependent concentration. PubMed: 22393054 |
WB
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion. -
Tissue specificity
Ubiquitous. Highest levels where found in placenta. -
Sequence similarities
Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
Cellular localization
Cell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 8773 Human
- Entrez Gene: 20619 Mouse
- Entrez Gene: 64630 Rat
- Omim: 602534 Human
- SwissProt: O00161 Human
- SwissProt: O09044 Mouse
- SwissProt: O70377 Rat
- Unigene: 511149 Human
see all -
Alternative names
- HsT17016 antibody
- LS-B8340 antibody
- SNAP 23 antibody
see all
Images
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IHC image of ab4114 staining in human placenta tissue formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4114, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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Datasheet download
References (22)
ab4114 has been referenced in 22 publications.
- Brasher MI et al. Syntaxin4-Munc18c Interaction Promotes Breast Tumor Invasion and Metastasis by Regulating MT1-MMP Trafficking. Mol Cancer Res 20:434-445 (2022). PubMed: 34876482
- Hu CY et al. Long non-coding RNA NORAD promotes the prostate cancer cell extracellular vesicle release via microRNA-541-3p-regulated PKM2 to induce bone metastasis of prostate cancer. J Exp Clin Cancer Res 40:98 (2021). PubMed: 33722248
- Qian L et al. Reduced O-GlcNAcylation of SNAP-23 promotes cisplatin resistance by inducing exosome secretion in ovarian cancer. Cell Death Discov 7:112 (2021). PubMed: 34001861
- Sikorra S et al. Engineering an Effective Human SNAP-23 Cleaving Botulinum Neurotoxin A Variant. Toxins (Basel) 12:N/A (2020). PubMed: 33352834
- Xiong X et al. Acid Sphingomyelinase regulates the localization and trafficking of palmitoylated proteins. Biol Open 8:N/A (2019). PubMed: 31142470