SNAP25 ELISA Kit (ab128572)
- Product nameSNAP25 ELISA Kit
Intra-assay Sample n Mean SD CV% Overall 8 8%
- Tests1 x 96 well plate
- Sample typeTissue Extracts, Cell Lysate
- Assay typeSandwich
- Sensitivity> 58.9 pg/ml
Sample specific recovery Sample type Average % Range Serum 90 74% - 101% Cell culture extracts 71 61% - 79%
- Species reactivityReacts with: Mouse, Rat, Human
- Product overview
Synaptosomal-Associated 25 kD Protein, or SNAP25, was first identified as a neuron-specific gene expressed in mouse hippocampus. SNAP25 is a membrane protein that normally faces the cytoplasm. SNAP25 is a member of the t-SNARE family and associates reversibly with another t-SNARE, Syntaxin, in the presence of Ca2+. Cleavage of SNAP25 by botulinum neurotoxin A and/or E abrogates Ca2+ induced insulin secretion. Only the N-terminal SNARE motif of SNAP25 is necessary for formation of the SNAP25/Syntaxin complex. In conjunction with the rate limiting v-SNARE, Synaptobrevin, the SNAP25/Syntaxin complex is sufficient for vesicle docking and membrane fusion.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Blocking Buffer 1 x 6ml 10X HRP Label 1 x 1ml 10X SNAP25 Detector Antibody 1 x 700µl 20X Buffer 1 x 20ml Extraction Buffer 1 x 15ml Human SNAP25 Standard 1 x 1µg SNAP25 Microplate 1 unit TMB Development Solution 1 x 12ml
- SNAPSNAP-25SUPSuper proteinSynaptosomal-associated 25 kDa proteinSynaptosomal-associated protein 25Synaptosomal-associated protein, 25kDa
Our Abpromise guarantee covers the use of ab128572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SNAP25 ELISA Kit images
Example standard curve of serially diluted recombinant SNAP25 protein (ab74529) in the working range of the assay (0.06 – 50 ng/mL).
Example Mouse Brain Homogenate dilution series in the working range of the assay.
Sample recovery under 3 different conditions compared with the wash buffer from the kit. 10% NGS and 50% RIPA buffer decrease the signal recovery by approximately 20-25% on average.
The detector antibody used in this kit specifically detects human SNAP25 recombinant protein (lane 2) as determined by Western Blotting. This antibody is species cross reactive for SNAP25 in Mouse Brain Homogenate (lane 3), Rat Brain Homogenate (lane 5), and Human Brain Homogenate (lane 7). SNAP25 is specifically expressed in brain tissue as indicated by a lack of signal in Mouse Heart Homogenate (lane 4), Rat Heart Homogenate (lane 6), Human Heart Homogenate (lane 8).
Example Assay Tissue Specificity with Mouse Heart Homogenate (MHH), Human Heart Homogenate (HHH), Rat Heart Homogenate (RHH), Mouse Brain Homogenate (MBH), and Rat Brain Homogenate (RBH).
References for SNAP25 ELISA Kit (ab128572)
ab128572 has not yet been referenced specifically in any publications.