For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
|Sample type||Average %||Range|
|Serum||90||74% - 101%|
|Cell culture extracts||71||61% - 79%|
Synaptosomal-Associated 25 kD Protein, or SNAP25, was first identified as a neuron-specific gene expressed in mouse hippocampus. SNAP25 is a membrane protein that normally faces the cytoplasm. SNAP25 is a member of the t-SNARE family and associates reversibly with another t-SNARE, Syntaxin, in the presence of Ca2+. Cleavage of SNAP25 by botulinum neurotoxin A and/or E abrogates Ca2+ induced insulin secretion. Only the N-terminal SNARE motif of SNAP25 is necessary for formation of the SNAP25/Syntaxin complex. In conjunction with the rate limiting v-SNARE, Synaptobrevin, the SNAP25/Syntaxin complex is sufficient for vesicle docking and membrane fusion.
|Components||1 x 96 tests|
|10X Blocking Buffer||1 x 6ml|
|10X HRP Label||1 x 1ml|
|10X SNAP25 Detector Antibody||1 x 700µl|
|20X Buffer||1 x 20ml|
|Extraction Buffer||1 x 15ml|
|Human SNAP25 Standard||1 x 1µg|
|SNAP25 Microplate||1 unit|
|TMB Development Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab128572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab128572 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"