Overview

  • Product nameSNAP25 ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 8%
    Overall 8 8%
  • Tests
    1 x 96 well plate
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay typeSandwich (quantitative)
  • Sensitivity
    > 58.9 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 90 74% - 101%
    Cell culture extracts 71 61% - 79%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Synaptosomal-Associated 25 kD Protein, or SNAP25, was first identified as a neuron-specific gene expressed in mouse hippocampus. SNAP25 is a membrane protein that normally faces the cytoplasm. SNAP25 is a member of the t-SNARE family and associates reversibly with another t-SNARE, Syntaxin, in the presence of Ca2+. Cleavage of SNAP25 by botulinum neurotoxin A and/or E abrogates Ca2+ induced insulin secretion. Only the N-terminal SNARE motif of SNAP25 is necessary for formation of the SNAP25/Syntaxin complex. In conjunction with the rate limiting v-SNARE, Synaptobrevin, the SNAP25/Syntaxin complex is sufficient for vesicle docking and membrane fusion.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceSynaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.
  • Cellular localizationCytoplasm, perinuclear region. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Note: Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
  • Alternative names
    • RIC-4
    • RIC4
    • SEC9
    • SNAP
    • SNAP-25
    • SUP
    • Super protein
    • Synaptosomal-associated 25 kDa protein
    • Synaptosomal-associated protein 25
    • Synaptosomal-associated protein, 25kDa
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab128572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

SNAP25 ELISA Kit images

  • Example standard curve of serially diluted recombinant SNAP25 protein (ab74529) in the working range of the assay (0.06 – 50 ng/mL).
  • Example Mouse Brain Homogenate dilution series in the working range of the assay.
  • Sample recovery under 3 different conditions compared with the wash buffer from the kit. 10% NGS and 50% RIPA buffer decrease the signal recovery by approximately 20-25% on average.
  • The detector antibody used in this kit specifically detects human SNAP25 recombinant protein (lane 2) as determined by Western Blotting. This antibody is species cross reactive for SNAP25 in Mouse Brain Homogenate (lane 3), Rat Brain Homogenate (lane 5), and Human Brain Homogenate (lane 7). SNAP25 is specifically expressed in brain tissue as indicated by a lack of signal in Mouse Heart Homogenate (lane 4), Rat Heart Homogenate (lane 6), Human Heart Homogenate (lane 8).
  • Example Assay Tissue Specificity with Mouse Heart Homogenate (MHH), Human Heart Homogenate (HHH), Rat Heart Homogenate (RHH), Mouse Brain Homogenate (MBH), and Rat Brain Homogenate (RBH).

Protocols

References for SNAP25 ELISA Kit (ab128572)

ab128572 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"