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Synthetic peptide: C-DEANQRATKMLGSG, corresponding to C terminal amino acids 193-206 of Human SNAP25
Our Abpromise guarantee covers the use of ab31281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 - 4 µg/ml.|
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
ICC/IF image of ab31281 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31281 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab31281 at 2.5µg/ml staining SNAP25 in human cerebral cortex tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
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