Anti-SNAP25 antibody (ab41455)
Key features and details
- Rabbit polyclonal to SNAP25
- Suitable for: IP, ICC, WB
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SNAP25 antibody
See all SNAP25 primary antibodies -
Description
Rabbit polyclonal to SNAP25 -
Host species
Rabbit -
Specificity
Replenishment batches of our polyclonal antibody, ab41455 are tested in WB. Previous batches were additionally validated in ICC and IP. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab109105.
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Tested applications
Suitable for: IP, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Zebrafish
Predicted to work with: Chicken, Cow -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use a concentration of 5 µg/ml.
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ICC |
Use a concentration of 1 - 5 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
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Notes |
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IP
Use a concentration of 5 µg/ml. |
ICC
Use a concentration of 1 - 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa). |
Target
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Function
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. -
Tissue specificity
Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. -
Sequence similarities
Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
Post-translational
modificationsPalmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. -
Cellular localization
Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 396444 Chicken
- Entrez Gene: 540853 Cow
- Entrez Gene: 6616 Human
- Entrez Gene: 20614 Mouse
- Entrez Gene: 25012 Rat
- Entrez Gene: 30712 Zebrafish
- Omim: 600322 Human
- SwissProt: P60878 Chicken
see all -
Alternative names
- bA416N4.2 antibody
- Bdr antibody
- CMS18 antibody
see all
Images
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ab41455 staining SNAP25 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab41455 at 1µg/ml and ab192757, Mouse mono Anti-PSD95 antibody [K28/43] - Synaptic Marker. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-SNAP25 antibody (ab41455) at 1 µg/ml
Lane 1 : Spinal cord (Rat) tissue lysate
Lane 2 : Hippocampus (Mouse) tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted? -
ab4455 staining SNAP25 in PC12 cells. The cells were fixed with 100% methanol (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab41455 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
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All lanes : Anti-SNAP25 antibody (ab41455) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : SH-SY5Y (Human neuroblastoma cell line) whole cell lysate at 20 µg
Lane 4 : Mouse brain homogenate at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes -
SNAP25 was immunoprecipitated using 0.5mg Rat Spinal Cord tissue lysate, 5µg of Rabbit polyclonal to SNAP25 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Rat Spinal Cord tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab41455.
Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
Band: 26kDa; SNAP25
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab41455 has been referenced in 18 publications.
- Yeh KC et al. Soybean Meal Extract Preserves Memory Ability by Increasing Presynaptic Function and Modulating Gut Microbiota in Rats. Mol Neurobiol 59:1649-1664 (2022). PubMed: 35001354
- Lu CW et al. Asiatic Acid Prevents Cognitive Deficits by Inhibiting Calpain Activation and Preserving Synaptic and Mitochondrial Function in Rats with Kainic Acid-Induced Seizure. Biomedicines 9:N/A (2021). PubMed: 33802221
- Ingwersen T et al. G392E neuroserpin causing the dementia FENIB is secreted from cells but is not synaptotoxic. Sci Rep 11:8766 (2021). PubMed: 33888787
- Shumate KM et al. RNA editing-mediated regulation of calcium-dependent activator protein for secretion (CAPS1) localization and its impact on synaptic transmission. J Neurochem 158:182-196 (2021). PubMed: 33894004
- Comella-Bolla A et al. Human Pluripotent Stem Cell-Derived Neurons Are Functionally Mature In Vitro and Integrate into the Mouse Striatum Following Transplantation. Mol Neurobiol 57:2766-2798 (2020). PubMed: 32356172