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Overview

  • Product nameSNAP47 protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 231-378 of Human SNAP47 with N terminal His tag; 148 amino acids, 24kDa. UniProt ID = Q5SQN1.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 55 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesFIGKPDMAYRLISAKMPEVIPILEVQFSKKMELLEDALVL RSARTSSPAEKSCSV WHAASGLMGRTLHREPPAGDQEG TALHLQTSLPALSEADTQELTQILRRMKGLAL EAESEL ERQDEALDGVAAAVDRATLTIDKHNRRMKRLT
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91855 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        C1orf142Chromosome 1 open reading frame 142FLJ12517
        SNAP 47SVAP1t SNARE coiled coil homology domain containing protein C1orf142
      see all
  • RelevanceSNAP47 belongs to the SVAP1 family. It contains two t-SNARE coiled-coil homology domains. SNAP47 has ubiquitous tissue distribution, with particularly high levels in nervous tissue. In neurons, SNAP 47 has been shown to have widespread distribution on intracellular membranes and is enriched in synaptic vesicle fractions. In vitro, SNAP 47 can substitute for SNAP-25 in SNARE complex formation, but not as efficiently.
  • Cellular localizationEndomembrane system. Cytoplasm; perinuclear region. Note: Appears to be exclusively membrane-bound.

    • SNAP47 protein (Tagged-His Tag) images

    • SDS-PAGE - SNAP47 protein (Tagged-His Tag) (ab91855)
      SDS-PAGE - SNAP47 protein (Tagged-His Tag) (ab91855)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for SNAP47 protein (Tagged-His Tag) (ab91855)

    ab91855 has not yet been referenced specifically in any publications.

    Publishing research using ab91855 ? Please let us know so that we can cite the reference in this datasheet

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