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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-SOCS1 antibody
See all SOCS1 products (7) ...
Description
Rabbit polyclonal to SOCS1
Specificity
By Western blot, this antibody detects a band of approximately 24 kDa in GH treated HEK 293 cell lysate, which corresponds to the predicted molecular weight of SOCS 1. However, in human spleen cell lysate a band at 37kDa is observed.
Tested applications
ICC/IF, Flow Cyt, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: VLRDYLSSFPFQI, corresponding to amino acids 199-211 of human SOCS 1 or 200-212 of mouse SOCS 1.
VLRDYLSSFP FQI
Positive control
human spleen lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Immunology >> Immune System Diseases >> Autoimmune
Signal Transduction >> Adapters >> Cytoplasmic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
Flow Cyt: Use at an assay dependent dilution. (PubMed: 19435411)
WB: Use a concentration of 1 - 4 µg/ml.Detects a band of approximately 38 kDa (predicted molecular weight: 24 kDa).
Target
Function
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2.
Tissue specificity
Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 1 SH2 domain.
Contains 1 SOCS box domain.
Domain
The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box.
Target information above from: UniProt accessionO15524
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CISH 1 antibody
- CISH1 antibody
- CISH1 antibody
see all
Database links
- Entrez Gene: 8651 Human
- Entrez Gene: 12703 Mouse
- Omim: 603597 Human
- SwissProt: O15524 Human
- SwissProt: O35716 Mouse
- Unigene: 50640 Human
- Unigene: 130 Mouse
Anti-SOCS1 antibody images:
Western blot - SOCS1 antibody (ab3691)
Lane 1 : Anti-SOCS1 antibody (ab3691) at 1 µg/ml
Lane 2 : Anti-SOCS1 antibody (ab3691) at 2 µg/ml
Lane 3 : Anti-SOCS1 antibody (ab3691) at 4 µg/ml
Lane 1 : human spleen lysate
Lane 2 : human spleen lysate
Lane 3 : human spleen lysate
Predicted band size : 24 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence-SOCS1 antibody(ab3691)
ICC/IF image of ab3691 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3691, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-SOCS1 antibody (ab3691)
This product has been referenced in:
- Yin SYet al. Interleukin-4 enhances trafficking and functional activities of GM-CSF-stimulated mouse myeloid-derived dendritic cells at late differentiation stage. Exp Cell Res 317:2210-21 (2011). WB; Mouse.Read more (PubMed: 21741972) »
- Carlier Jet al. Paracrine inhibition of GM-CSF signaling by human cytomegalovirus in monocytes differentiating to dendritic cells. Blood 118:6783-92 (2011). WB.Read more (PubMed: 22031867) »
See all 13 publications for this product
Publishing research using ab3691? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"