Anti-SOCS1 antibody (ab62584)
Key features and details
- Rabbit polyclonal to SOCS1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SOCS1 antibody
See all SOCS1 primary antibodies -
Description
Rabbit polyclonal to SOCS1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic 15 amino acid peptide from near the carboxy terminus of human SOCS1.
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Positive control
- WB: Human spleen cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 24 kDa).
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 24 kDa). |
Target
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Function
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. -
Tissue specificity
Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
Domain
The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box. - Information by UniProt
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Database links
- Entrez Gene: 8651 Human
- Omim: 603597 Human
- SwissProt: O15524 Human
- Unigene: 50640 Human
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Alternative names
- CIS1 antibody
- CISH 1 antibody
- CISH1 antibody
see all
Images
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Lane 1 : Anti-SOCS1 antibody (ab62584) at 1 µg/ml
Lane 2 : Anti-SOCS1 antibody (ab62584) at 2 µg/ml
Lane 3 : Anti-SOCS1 antibody (ab62584) at 4 µg/ml
All lanes : Human spleen
cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 24 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab62584 has been referenced in 26 publications.
- Zhang Z et al. Macrophage‑derived exosomal miRNA‑155 promotes tubular injury in ischemia‑induced acute kidney injury. Int J Mol Med 50:N/A (2022). PubMed: 35795997
- Wu H et al. Mer regulates microglial/macrophage M1/M2 polarization and alleviates neuroinflammation following traumatic brain injury. J Neuroinflammation 18:2 (2021). PubMed: 33402181
- Yang J et al. The inhibitory effect of Gualou Guizhi Decoction on post-ischemic neuroinflammation via miR-155 in MCAO rats. Ann Palliat Med 10:1370-1379 (2021). PubMed: 33081480
- Liu W et al. Long Non-Coding RNA NORAD Inhibits Breast Cancer Cell Proliferation and Metastasis by Regulating miR-155-5p/SOCS1 Axis. J Breast Cancer 24:330-343 (2021). PubMed: 34190442
- Huang Y et al. GFI1 promotes the proliferation and migration of esophageal squamous cell carcinoma cells through the inhibition of SOCS1 expression. Int J Mol Med 48:N/A (2021). PubMed: 34368875