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Products:Immunology >> Innate Immunity >> Cytokines >> IL6 Related
Overview
Product name
Anti-SOCS3 antibody
See all SOCS3 products (12) ...
Description
Rabbit polyclonal to SOCS3
Tested applications
ICC/IF, Flow Cyt, WB, IHC-Fr, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Cow, Dog
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human SOCS3.
(Peptide available as ab16199.)
Positive control
This antibody gave a positive signal in the following Human Whole Cell Lysates: Hela, Jurkat, A431 and MOLT4.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Signal Transduction >> Adapters >> Cytoplasmic
Immunology >> Innate Immunity >> Cytokines >> IL6 Related
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
Flow Cyt: 1/300
WB: Use a concentration of 1 µg/mlDetects a band of approximately 30 kDa (predicted molecular weight: 24.7 kDa).Can be blocked with SOCS3 peptide (ab16199).
IHC-Fr: Use at an assay dependent dilution. (PubMed: 19855398)
IHC-P: 1/100Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Target
Function
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
Tissue specificity
Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
Pathway
Protein modification; protein ubiquitination.
Involvement in disease
Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
Sequence similarities
Contains 1 SH2 domain.
Contains 1 SOCS box domain.
Domain
The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
Post-translational
modifications
Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
Target information above from: UniProt accessionO14543
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ATOD4 antibody
- CIS 3 antibody
- CIS-3 antibody
see all
Database links
- Entrez Gene: 282081 Cow
- Entrez Gene: 442949 Dog
- Entrez Gene: 9021 Human
- Entrez Gene: 12702 Mouse
- Entrez Gene: 89829 Rat
- Omim: 604176 Human
- SwissProt: Q90X67 Chicken
- SwissProt: Q9BEG9 Cow
see all
Anti-SOCS3 antibody images:
Western blot - SOCS3 antibody (ab16030)
All lanes : Anti-SOCS3 antibody (ab16030) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with SOCS3 peptide (ab16199) at 1 µg/ml
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with SOCS3 peptide (ab16199) at 1 µg/ml
Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with SOCS3 peptide (ab16199) at 1 µg/ml
Lane 8 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate (ab52252) with SOCS3 peptide (ab16199) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
The 30kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SOCS3.
Immunocytochemistry/ Immunofluorescence - SOCS3 antibody (ab16030)
ICC/IF image of ab16030 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab16030, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOCS3 antibody (ab16030)
Image courtesy of Human Protein Atlas
ab16030 staining SOCS3 in human bone marrow showing strong staining within the bone marrow. Paraffin embedded human bone marrow was incubated with ab16030 (1/100) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH6.
ab16030 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Immunohistochemistry (Frozen sections) - SOCS3 antibody (ab16030)
ab16030 staining SOCS3 in Human pancreatic tumour tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/300 in PBS) for 8 hours at 4°C. An Alexa Fluor®555-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were stained by DAPI.
This image is courtesy of an anonymous Abreview
References for Anti-SOCS3 antibody (ab16030)
This product has been referenced in:
- Wang Yet al. Elevated maternal soluble Gp130 and IL-6 levels and reduced Gp130 and SOCS-3 expressions in women complicated with preeclampsia. Hypertension 57:336-42 (2011).Read more (PubMed: 21173340) »
- Ghanim Het al. A resveratrol and polyphenol preparation suppresses oxidative and inflammatory stress response to a high-fat, high-carbohydrate meal. J Clin Endocrinol Metab 96:1409-14 (2011). Human.Read more (PubMed: 21289251) »
See all 9 publications for this product
Publishing research using ab16030? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"