Overview

  • Product nameAnti-SOCS3 antibodySee all SOCS3 primary antibodies ...
  • Description
    Goat polyclonal to SOCS3
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: PIREFLDQYDAPL, corresponding to C terminal amino acids 213-225 of Human SOCS3 .

  • Positive control
    • MOLT-4 lysate
  • General notesPrincipal Names - SOCS3; CIS3; Cish3; SSI-3; SOCS-3; suppressor of cytokine signaling 3; STAT induced STAT inhibitor 3; cytokine-induced SH2 protein 3 Official Gene Symbol - SOCS3 GenBank Accession Number – NP_003946 LocusLink ID - 9021 (human); 12702 (mouse); 89829 (rat) Gene Ontology terms - protein kinase inhibitor; JAK-STAT cascade; anti-apoptosis Size - 225 aa

Properties

Applications

Our Abpromise guarantee covers the use of ab723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWestern Blot - Approx 24kDa band seen in MOLT-4 lysate. Recommended for use at 4.0-6.0µg/ml. Block membrane in 2.5% skimmed milk in TBST (TBS + 0.05% Tween). Incubate primary antibody for 1 hour at room temperature with agitation.
  • Target

    • FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
    • Tissue specificityWidely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
    • PathwayProtein modification; protein ubiquitination.
    • Involvement in diseaseNote=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
    • Sequence similaritiesContains 1 SH2 domain.
      Contains 1 SOCS box domain.
    • DomainThe ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
      The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATOD4 antibody
      • CIS 3 antibody
      • CIS-3 antibody
      • CIS3 antibody
      • Cish3 antibody
      • Cytokine induced SH2 protein 3 antibody
      • Cytokine-inducible SH2 protein 3 antibody
      • E2a Pbx1 target gene in fibroblasts 10 antibody
      • EF 10 antibody
      • MGC71791 antibody
      • SOCS 3 antibody
      • SOCS-3 antibody
      • Socs3 antibody
      • SOCS3_HUMAN antibody
      • SSI 3 antibody
      • SSI-3 antibody
      • SSI3 antibody
      • STAT induced STAT inhibitor 3 antibody
      • STAT-induced STAT inhibitor 3 antibody
      • Suppressor of cytokine signaling 3 antibody
      see all

    Anti-SOCS3 antibody images

    References for Anti-SOCS3 antibody (ab723)

    ab723 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your email. Ab723 is 325 Euros and is in stock in our office in Great Britain so upon ordering, you should receive it within a couple of days. You can place your order online, or via fax, email or phone. Below is the appropriate contact d...

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    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with rat has no...

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    Thank you for the details that you have provided. We have had no other complaints concerning this antibody. The antibody's originator has provided me with the following protocol that they used when testing the antibody which should be helpful for you: ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"