• Product name
  • Description
    Goat polyclonal to SOCS3
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: PIREFLDQYDAPL, corresponding to C terminal amino acids 213-225 of Human SOCS3 .

  • Positive control
    • MOLT-4 lysate
  • General notes
    Principal Names - SOCS3; CIS3; Cish3; SSI-3; SOCS-3; suppressor of cytokine signaling 3; STAT induced STAT inhibitor 3; cytokine-induced SH2 protein 3 Official Gene Symbol - SOCS3 GenBank Accession Number – NP_003946 LocusLink ID - 9021 (human); 12702 (mouse); 89829 (rat) Gene Ontology terms - protein kinase inhibitor; JAK-STAT cascade; anti-apoptosis Size - 225 aa



Our Abpromise guarantee covers the use of ab723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Can be blocked with Human SOCS3 peptide (ab22776).
Approx 24kDa band seen in MOLT-4 lysate. Recommended for use at 4.0-6.0µg/ml. Block membrane in 2.5% skimmed milk in TBST (TBS + 0.05% Tween). Incubate primary antibody for 1 hour at room temperature with agitation.



  • Function
    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
  • Tissue specificity
    Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
  • Sequence similarities
    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • Domain
    The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • Post-translational
    Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATOD4 antibody
    • CIS 3 antibody
    • CIS-3 antibody
    • CIS3 antibody
    • Cish3 antibody
    • Cytokine induced SH2 protein 3 antibody
    • Cytokine-inducible SH2 protein 3 antibody
    • E2a Pbx1 target gene in fibroblasts 10 antibody
    • EF 10 antibody
    • MGC71791 antibody
    • SOCS 3 antibody
    • SOCS-3 antibody
    • Socs3 antibody
    • SOCS3_HUMAN antibody
    • SSI 3 antibody
    • SSI-3 antibody
    • SSI3 antibody
    • STAT induced STAT inhibitor 3 antibody
    • STAT-induced STAT inhibitor 3 antibody
    • Suppressor of cytokine signaling 3 antibody
    see all


  • Anti-SOCS3 antibody (ab723) at 5 µg/ml + MOLT-4 lysate (RIPA buffer, 30µg total protein per lane)

    Observed band size: 24 kDa (why is the actual band size different from the predicted?)

    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.


This product has been referenced in:
  • Minter MR  et al. Deletion of the type-1 interferon receptor in APPSWE/PS1?E9 mice preserves cognitive function and alters glial phenotype. Acta Neuropathol Commun 4:72 (2016). WB . Read more (PubMed: 27400725) »

See 1 Publication for this product

Customer reviews and Q&As

Western blot
Mouse Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (3)
Loading amount
45 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 21 2017

Thank you for your email. Ab723 is 325 Euros and is in stock in our office in Great Britain so upon ordering, you should receive it within a couple of days. You can place your order online, or via fax, email or phone. Below is the appropriate contact d...

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Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with rat has no...

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Thank you for the details that you have provided. We have had no other complaints concerning this antibody. The antibody's originator has provided me with the following protocol that they used when testing the antibody which should be helpful for you: ...

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