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Overview

  • Product nameAnti-SOCS3 antibodySee all SOCS3 primary antibodies ...
  • Description
    Goat polyclonal to SOCS3
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: PIREFLDQYDAPL, corresponding to C terminal amino acids 213-225 of Human SOCS3 .

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlMOLT-4 lysate
  • General notesPrincipal Names - SOCS3; CIS3; Cish3; SSI-3; SOCS-3; suppressor of cytokine signaling 3; STAT induced STAT inhibitor 3; cytokine-induced SH2 protein 3 Official Gene Symbol - SOCS3 GenBank Accession Number – NP_003946 LocusLink ID - 9021 (human); 12702 (mouse); 89829 (rat) Gene Ontology terms - protein kinase inhibitor; JAK-STAT cascade; anti-apoptosis Size - 225 aa

Properties

Applications

Our Abpromise guarantee covers the use of ab723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWestern Blot - Approx 24kDa band seen in MOLT-4 lysate. Recommended for use at 4.0-6.0µg/ml. Block membrane in 2.5% skimmed milk in TBST (TBS + 0.05% Tween). Incubate primary antibody for 1 hour at room temperature with agitation.

    • Target

    • FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
    • Tissue specificityWidely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
    • PathwayProtein modification; protein ubiquitination.
    • Involvement in diseaseNote=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
    • Sequence similaritiesContains 1 SH2 domain.
      Contains 1 SOCS box domain.
    • DomainThe ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
      The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
    • Post-translational
      modifications      Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.

      • Target information above from: UniProt accession O14543 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        ATOD4 antibodyCIS 3 antibodyCIS-3 antibody
        CIS3 antibodyCish3 antibodyCytokine induced SH2 protein 3 antibodyCytokine-inducible SH2 protein 3 antibodyE2a Pbx1 target gene in fibroblasts 10 antibodyEF 10 antibodyMGC71791 antibodySOCS 3 antibodySOCS-3 antibodySocs3 antibodySOCS3_HUMAN antibodySSI 3 antibodySSI-3 antibodySSI3 antibodySTAT induced STAT inhibitor 3 antibodySTAT-induced STAT inhibitor 3 antibodySuppressor of cytokine signaling 3 antibody
      see all

    Anti-SOCS3 antibody images

    References for Anti-SOCS3 antibody (ab723)

    ab723 has not yet been referenced specifically in any publications.

    Publishing research using ab723 ? Please let us know so that we can cite the reference in this datasheet

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    We have decided to order the antibody SOCS 3 ( cat.no. ab723 ) and hopefully we have a good staining on the rat sections. Can you tell me the price in euro's ( + taxes )? Is there something known about the deliverytime?

    Thank you for your email. Ab723 is 325 Euros and is in stock in our office in Great Britain so upon ordering, you should receive it within a couple of days. You can place your order online, or via fax, email or phone. Below is the appropriate contact de...

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    We are interested in the SOCS 3 antibody ( cat.no. ab 723 ). I have some questions: Does this antibody work on rat material? We are using fresh or paraformaldehyde fixed rat brain tissue. The sections are vibratome sections and the technique is IHC.

    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with rat has no...

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    Here is the answer to your questions. 1.High backgroung, several bands trouhgout the blot. The positive control did not show in the blot ( molt 4 whole cell lysate) 2.Human left ventricle, total protein 3.45ug of total protein. I used a 2% SDS lysis ...

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    Thank you for the details that you have provided. We have had no other complaints concerning this antibody. The antibody's originator has provided me with the following protocol that they used when testing the antibody which should be helpful for you: ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"