Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
SOD2 Human ELISA Kit
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 3.6% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| 1 | 3 | 13% |
Sample type
Cell culture extracts, Tissue Extracts
Assay type
Sandwich
Sensitivity
4 ng/ml
Range
4 ng/ml - 500 ng/ml
Recovery
103%
| Sample type | Average % | Range % |
|---|---|---|
| Serum | 103 | 101 - 105 |
Cross reactivity
Reacts with
Human
Notes
The principle cellular anti-oxidant enzymes are the superoxide dismutase family (SOD, E.C. 1.15.1.1). These enzymes dismutate superoxide into hydrogen peroxide which is further detoxified by other cellular defenses such as glutathione peroxidase and catalase. Superoxide and its products have been implicated in a wide range of diseases including cancer, inflammation, neurodegenerative diseases, diabetes and aging itself.
The SOD family has 3 members, two of which are Cu-Zn type - the extracellular SOD3 and the cytoplasmic SOD1. The other member is the mitochondrial Mn (manganese) type SOD2. The mitochondrial Mn-SOD2 is a homotetramer of subunit mass 23 kDa in the mitochondrial matrix. SOD2 has been shown to be essential since knockout mice die shortly after birth. SOD2 levels may be down-regulated in tumor cells and recent observations show that over expression of SOD2 in tumor cells may suppress cell division and cancer growth (Oberley, Biomedecine & Pharmacotherapy, 2005, 59, p143-8).
Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
Storage: Store all components at 4°C. This kit is stable for at least 6 months from receipt. After reconstitution the standard should be stored at -80°C. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
The SOD family has 3 members, two of which are Cu-Zn type - the extracellular SOD3 and the cytoplasmic SOD1. The other member is the mitochondrial Mn (manganese) type SOD2. The mitochondrial Mn-SOD2 is a homotetramer of subunit mass 23 kDa in the mitochondrial matrix. SOD2 has been shown to be essential since knockout mice die shortly after birth. SOD2 levels may be down-regulated in tumor cells and recent observations show that over expression of SOD2 in tumor cells may suppress cell division and cancer growth (Oberley, Biomedecine & Pharmacotherapy, 2005, 59, p143-8).
Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
Storage: Store all components at 4°C. This kit is stable for at least 6 months from receipt. After reconstitution the standard should be stored at -80°C. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 10X HRP Label | P/N 8206020 | 1 x 1ml |
| 10X Blocking Buffer | P/N 8209802 | 1 x 6ml |
| 10X Detector Antibody | P/N 8209532 | 1 x 1ml |
| 20X Buffer | P/N 8203013 | 1 x 20ml |
| Development Solution | P/N 8209803 | 1 x 12ml |
| Extraction Buffer | P/N 8201093 | 1 x 15ml |
| Human SOD2 Protein Standard (1 µg) ab84639 | P/N 8209520 | 1 vial |
| SOD2 Microplate | P/N 8209519 | 1 unit |
Research areas
Metabolism >> Redox metbolism >> Antioxidants
Metabolism >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
Relevance
This is a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Cellular localization
Mitochondrial
Alternative names
- IPOB
- Manganese superoxide dismutase
- SOD2
see all
Database links
- Entrez Gene: 6648 Human
- Omim: 147460 Human
- SwissProt: P04179 Human
- Unigene: 487046 Human
SOD2 Human ELISA Kit images:
ELISA - SOD2 Human ELISA Kit (ab119694)
Figure 1. An example of a dilution series of recombinant SOD2 in the working range of the assay kit.
ELISA - SOD2 Human ELISA Kit (ab119694)
Figure 2. Example HeLa dilution series in the working range of the SOD2 ELISA kit.
ELISA - SOD2 Human ELISA Kit (ab119694)
Figure 3. The expression level of SOD2 varies by cell type. Four cell types are analyzed by SOD2 expression level relative to the human HepG2 cell line.
Western blot - SOD2 Human ELISA Kit (ab119694)
Figure 4. The relative relationship of various expression levels is confirmed by Western blotting using the Abcam antibody ab13533.
Immunocytochemistry/ Immunofluorescence - SOD2 Human ELISA Kit (ab119694)
Figure 5. The SOD2 detector antibody in this ELISA is available as standalone antibody ab110300. Immunocytochemistry image of Abcam ab110300 stained human HDFn cells. Target protein localizes to the mitochondria.
Protocols
References for SOD2 Human ELISA Kit (ab119694)
ab119694 has not yet been referenced specifically in any publications.
Publishing research using ab119694? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"