Recombinant Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPVANR2] to SOD2/MnSOD (acetyl K68)
- Suitable for: IHC-P, WB, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2]
See all SOD2/MnSOD primary antibodies -
Description
Rabbit monoclonal [EPVANR2] to SOD2/MnSOD (acetyl K68) -
Host species
Rabbit -
Specificity
This antibody only detects SOD2/MnSOD when acetylated at Lysine 68. According to BLAST analysis, the antibody might cross-react with Fer (Uniprot P70451) isoform 3 in mouse samples. No experiment has been done to confirm this possibility.
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Tested applications
Suitable for: IHC-P, WB, Dot blotmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
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Positive control
- WB: Mouse heart lysate. Rat kidney tissue lysate. SOD2 transfected HEK-293T lysate. IHC-P: Human cervical carcinoma. Human carcinoma kidney tissue. Rat liver tissue. Mouse kidney tissue. Human muscle tissue.
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General notes
This antibody was developed as part of a collaboration with the lab of David Guis at Vanderbilt University.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPVANR2 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (4) |
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
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Dot blot |
1/1000.
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Notes |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 24 kDa. |
Dot blot
1/1000. |
Target
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Function
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. -
Involvement in disease
Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
Sequence similarities
Belongs to the iron/manganese superoxide dismutase family. -
Post-translational
modificationsNitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 6648 Human
- Entrez Gene: 20656 Mouse
- Entrez Gene: 24787 Rat
- Omim: 147460 Human
- SwissProt: P04179 Human
- SwissProt: P09671 Mouse
- SwissProt: P07895 Rat
- Unigene: 487046 Human
see all -
Alternative names
- Indophenoloxidase B antibody
- IPO B antibody
- IPOB antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human clear cell carcinoma kidney tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (purified) + Mouse heart lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 24 kDaBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 24 kDaBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling SOD2 (acetyl K68) with purified ab137037 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling SOD2 (acetyl K68) with unpurified ab137037 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (unpurified)
Lane 1 : Non transfected HEK-293T lysate
Lane 2 : SOD2 transfected HEK-293T lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 24 kDa -
All lanes : Anti-SOD2/MnSOD (acetyl K68) antibody [EPVANR2] (ab137037) at 1/1000 dilution (unpurified)
Lane 1 : Non transfected HEK-293T cell lysate with with Acetyl SOD2 (K68) peptide
Lane 2 : SOD2 transfected HEK-293T cell lysate with control peptide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 24 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling SOD2 (acetyl K68) with unpurified ab137037 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Dot blot analysis labelling SOD2 (acetyl K68) with ab137037 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and diluting buffer: 5% NFDM/TBST.
Lane 1: SOD2 (acetyl K68) acetylated peptide.
Lane 2: SOD2 Non-acetylated peptide.
Exposure time: 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (81)
ab137037 has been referenced in 81 publications.
- Huang YM et al. Glucagon-Like Peptide-2 Ameliorates Age-Associated Bone Loss and Gut Barrier Dysfunction in Senescence-Accelerated Mouse Prone 6 Mice. Gerontology 69:428-449 (2023). PubMed: 36470214
- Chen DK et al. The bibenzyl derivatives of Dendrobium officinale prevent UV-B irradiation induced photoaging via SIRT3. Nat Prod Bioprospect 12:1 (2022). PubMed: 35084580
- Qin N et al. [Polydatin improves intestinal barrier injury after traumatic brain injury in rats by reducing oxidative stress and inflammatory response via activating SIRT1-mediated deacetylation of SOD2 and HMGB1]. Nan Fang Yi Ke Da Xue Xue Bao 42:93-100 (2022). PubMed: 35249875
- Peugnet V et al. Mitochondrial-Targeted Therapies Require Mitophagy to Prevent Oxidative Stress Induced by SOD2 Inactivation in Hypertrophied Cardiomyocytes. Antioxidants (Basel) 11:N/A (2022). PubMed: 35453408
- Wang W et al. NOX4 blockade suppresses titanium nanoparticle-induced bone destruction via activation of the Nrf2 signaling pathway. J Nanobiotechnology 20:241 (2022). PubMed: 35606794