Overview

  • Product nameAnti-SOD2 antibodySee all SOD2 primary antibodies ...
  • Description
    Sheep polyclonal to SOD2
  • SpecificityRecognizes the ~25 kDa SOD2 protein in human liver cell lysates. Does not cross react with Cu/Zn SOD or human serum.
  • Tested applicationsIHC-FoFr, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Hamster, Human
  • Immunogen

    Full length native protein from liver (purified) (Human)

  • Positive control
    • Human liver cell lysate

Applications

Our Abpromise guarantee covers the use of ab55835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
WB
IHC-Fr
IHC-P
  • Application notesIHC-Fr: 1/20 - 1/200.
    IHC-P: 1/20 - 1/200.
    IHC-FoFr: 1/1000 - 1/2000 on perfusion fixed tissues sectioned with a freezing microtome or cryostat. After sectioning, the sections can be stored overnight at 4C in 0.25 M Tris-HCl, pH 7.4, 0.9 NaCl containing 0.1% sodium azide.
    Notes:
    1. Quench endogenous peroxidase with 0.3% H2O2 (v/v).
    2. Perform blocking of nonspecific binding with 1:30 normal rabbit serum in 0.1% Triton X 100.
    3. Incubate sections in primary antibody at 4C for TWO days in 1% normal rabbit serum and 1% normal human serum in TBS with 0.01% thimerosal.
    4. Detect the immunoreactivity using horseradish peroxidase.

    WB: 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 16 kDa).

    Is unsuitable for DID.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDestroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
    • Involvement in diseaseGenetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
    • Sequence similaritiesBelongs to the iron/manganese superoxide dismutase family.
    • Post-translational
      modifications
      Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
    • Cellular localizationMitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Indophenoloxidase B antibody
      • IPO B antibody
      • IPOB antibody
      • Manganese containing superoxide dismutase antibody
      • Manganese SOD antibody
      • Manganese superoxide dismutase antibody
      • Mangano superoxide dismutase antibody
      • Mn SOD antibody
      • Mn superoxide dismutase antibody
      • MNSOD antibody
      • MVCD6 antibody
      • SOD 2 antibody
      • SOD2 antibody
      • SODM_HUMAN antibody
      • Superoxide dismutase [Mn] mitochondrial antibody
      • Superoxide dismutase [Mn], mitochondrial antibody
      • Superoxide dismutase 2 mitochondrial antibody
      see all

    References for Anti-SOD2 antibody (ab55835)

    ab55835 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab55835.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"