Anti-SOX10 antibody (ab180228)
Key features and details
- Goat polyclonal to SOX10
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-SOX10 antibody
See all SOX10 primary antibodies -
Description
Goat polyclonal to SOX10 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Human SOX10 aa 351-364 (internal sequence) (Cysteine residue). (NP_008872.1).
Sequence:DAKAQVKTETAGPQ
Database link: P56693 -
Positive control
- Human brain cerebellum lysate. Mouse brain lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab180228 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 50-52 kDa (predicted molecular weight: 49 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 50-52 kDa (predicted molecular weight: 49 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia. -
Tissue specificity
Expressed in fetal brain and in adult brain, heart, small intestine and colon. -
Involvement in disease
Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 353115 Cow
- Entrez Gene: 6663 Human
- Entrez Gene: 20665 Mouse
- Entrez Gene: 414903 Pig
- Entrez Gene: 29361 Rat
- Omim: 602229 Human
- SwissProt: P56693 Human
- SwissProt: Q04888 Mouse
see all -
Alternative names
- DOM antibody
- DOM antibody
- Dominant megacolon mouse human homolog of antibody
see all
Images
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Anti-SOX10 antibody (ab180228) at 0.1 µg/ml + Mouse brain lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?Primary incubation 1 hour at room temperature.
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Anti-SOX10 antibody (ab180228) at 0.1 µg/ml + Human brain cerebellum lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
Datasheets and documents
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Datasheet download
References (0)
ab180228 has not yet been referenced specifically in any publications.