Recombinant Anti-SOX11 antibody [EPR8192] (ab134107)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8192] to SOX11
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SOX11 antibody [EPR8192]
See all SOX11 primary antibodies -
Description
Rabbit monoclonal [EPR8192] to SOX11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain and Y79 lysates. IP: Y79 lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8192 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa).
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa). |
IP
1/10 - 1/100. |
Target
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Function
Probably important in the developing nervous system. May also have a role in tissue modeling during development. -
Tissue specificity
Expressed mainly in the nervous system. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6664 Human
- Omim: 600898 Human
- SwissProt: P35716 Human
- Unigene: 432638 Human
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Alternative names
- MRD27 antibody
- SOX11 antibody
- SOX11_HUMAN antibody
see all
Images
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All lanes : Anti-SOX11 antibody [EPR8192] (ab134107) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Y-79 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 47 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
SOX11 was immunoprecipitated from 0.35 mg Y79 (Human retinoblastoma retinoblastoma) whole cell lysate 10 µg with 134107 at 1/40 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Y79 (Human retinoblastoma retinoblastoma) whole cell lysate 10 µg
Lane 2: ab134107 IP in Y79 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab134107 in Y79 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab134107 has been referenced in 8 publications.
- Tsang SM et al. Sox11 regulates mammary tumour-initiating and metastatic capacity in Brca1-deficient mouse mammary tumour cells. Dis Model Mech 14:N/A (2021). PubMed: 33969421
- Yu Y et al. lncRNA PCNAP1 predicts poor prognosis in breast cancer and promotes cancer metastasis via miR-340-5p-dependent upregulation of SOX4. Oncol Rep 44:1511-1523 (2020). PubMed: 32945462
- Smestad JA & Maher LJ Master regulator analysis of paragangliomas carrying SDHx, VHL, or MAML3 genetic alterations. BMC Cancer 19:619 (2019). PubMed: 31234811
- Jacob A et al. The transcription factor prospero homeobox protein 1 is a direct target of SoxC proteins during developmental vertebrate neurogenesis. J Neurochem 146:251-268 (2018). PubMed: 29749639
- Ji Q et al. miR-223-3p Inhibits Human Osteosarcoma Metastasis and Progression by Directly Targeting CDH6. Mol Ther 26:1299-1312 (2018). PubMed: 29628305
- Balta EA et al. Phosphorylation Modulates the Subcellular Localization of SOX11. Front Mol Neurosci 11:211 (2018). PubMed: 29973868
- Tepe B et al. Single-Cell RNA-Seq of Mouse Olfactory Bulb Reveals Cellular Heterogeneity and Activity-Dependent Molecular Census of Adult-Born Neurons. Cell Rep 25:2689-2703.e3 (2018). PubMed: 30517858
- Balta EA et al. Phosphorylation of the neurogenic transcription factor SOX11 on serine 133 modulates neuronal morphogenesis. Sci Rep 8:16196 (2018). PubMed: 30385877