Anti-SOX17 antibody (ab83258)

Overview

• Product nameAnti-SOX17 antibodySee all SOX17 primary antibodies ...
• Description
  Rabbit polyclonal to SOX17
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Dog
• Immunogen

  Synthetic peptide corresponding to a region within internal sequence amino acids 360-409 (RTEFEQYLHF VCKPEMGLPY QGHDSGVNLP DSHGAISSVV SDASSAVYYC) of human SOX17 (NP_071899).

  Run BLAST with Run BLAST with
• Positive controlHeLa cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified by peptide affinity chromatography method.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Embryogenesis
  • Embryonic stem cells
  • Surface molecules

  • Stem Cells
  • Embryonic Stem Cells
  • Intracellular

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HMG Box

Applications

Target

• FunctionActs as transcription regulator that binds target promoter DNA and bends the DNA. Binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays a key role in the regulation of embryonic development. Required for normal looping of the embryonic heart tube. Required for normal development of the definitive gut endoderm. Probable transcriptional activator in the premeiotic germ cells.
• Tissue specificityExpressed in adult heart, lung, spleen, testis, ovary, placenta, fetal lung, and kidney. In normal gastrointestinal tract, it is preferentially expressed in esophagus, stomach and small intestine than in colon and rectum.
• Involvement in diseaseDefects in SOX17 are the cause of vesicoureteral reflux type 3 (VUR3) [MIM:613674]. VUR3 is a disease belonging to the group of congenital anomalies of the kidney and urinary tract. It is characterized by the reflux of urine from the bladder into the ureters and sometimes into the kidneys, and is a risk factor for urinary tract infections. Primary disease results from a developmental defect of the ureterovesical junction. In combination with intrarenal reflux, the resulting inflammatory reaction may result in renal injury or scarring, also called reflux nephropathy. Extensive renal scarring impairs renal function and may predispose patients to hypertension, proteinuria, renal insufficiency and end-stage renal disease.
• Sequence similaritiesContains 1 HMG box DNA-binding domain.
  Contains 1 Sox C-terminal domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q9H6I2 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 64321 Human
  • Entrez Gene: 20671 Mouse
  • Omim: 610928 Human
  • SwissProt: Q9H6I2 Human
  • SwissProt: Q61473 Mouse
  • Unigene: 98367 Human
  • Unigene: 279103 Mouse

• Alternative names
  FLJ22252 antibodySOX17 antibodySOX17_HUMAN antibody

  SRY (sex determining region Y) box 17 antibodySRY box 17 antibodySRY related HMG box transcription factor SOX17 antibodyTranscription factor SOX-17 antibodyTranscription factor SOX17 antibodyVUR3 antibody

  see all

Anti-SOX17 antibody images

• 

  Western blot - SOX17 antibody (ab83258)
  Anti-SOX17 antibody (ab83258) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg
  
  Secondary
  HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 44 kDa
  Observed band size : 44 kDa
  Additional bands at : 92 kDa. We are unsure as to the identity of these extra bands.