Recombinant Anti-SOX2 antibody [EPR3130] (ab133337)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3130] to SOX2
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SOX2 antibody [EPR3130]
See all SOX2 primary antibodies -
Description
Rabbit monoclonal [EPR3130] to SOX2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SOX2 aa 250-350. The exact sequence is proprietary.
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Positive control
- WB: NCCIT cell lysate; IHC: Human fetal brain, Human normal brain and glioma tissue. IP: NCCIT.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3130 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
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IP |
1/10.
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa). |
IP
1/10. |
Target
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Function
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. -
Involvement in disease
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Post-translational
modificationsSumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6657 Human
- Omim: 184429 Human
- SwissProt: P48431 Human
- Unigene: 518438 Human
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Alternative names
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
see all
Images
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Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human fetal brain tissue labelling SOX2 with ab133337 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human glioma tissue labelling SOX2 with ab133337 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human brain tissue staining SOX2 with ab133337 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Anti-SOX2 antibody [EPR3130] (ab133337) at 1/500 dilution + NCCIT cell lysate at 10 µg
Secondary
Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted? -
SOX2 was immunoprecipitated from 0.35 mg NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10 µg with 133337 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab133337 IP in NCCIT whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133337 in NCCIT whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab133337 has been referenced in 2 publications.
- Meneceur S et al. Establishment and Characterisation of Heterotopic Patient-Derived Xenografts for Glioblastoma. Cancers (Basel) 12:N/A (2020). PubMed: 32260145
- Liu Z et al. Specific marker expression and cell state of Schwann cells during culture in vitro. PLoS One 10:e0123278 (2015). WB, IF ; Mouse . PubMed: 25859851