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RabMAbs (2683-1)

Anti-SOX2 antibody [EPR3131] (ab92494)

Abreviews (2)Q&A (5)Specific References (4)

Overview

  • Product nameAnti-SOX2 antibody [EPR3131]See all SOX2 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR3131] to SOX2
  • Tested applicationsWB, ICC, Sandwich ELISA, IHC-P, IHC - Wholemount, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Human

    Does not react with

    Rat
  • Immunogen

    Synthetic peptide corresponding to residues in Human SOX2.

  • Positive controlNCCIT cell lysate, MCF-7 cell lysate, glioma tissue, Human adult brain tissue, Human fetal brain tissue
  • General notesProduced under U.S. Patent No. 5,675,063.

Applications

Our Abpromise guarantee covers the use of ab92494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
ICC ICC: 1/50 - 1/100.
Sandwich ELISA sELISA: Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [57CT23.3.4] to SOX2 (ab75485). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Rabbit monoclonal [EPR3131] to SOX2 (ab92494) as Capture.
IHC-P IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
IHC - Wholemount IHC - Wmt: Use at an assay dependent concentration.
ICC/IF ICC/IF: Use at an assay dependent concentration. PubMed: 22912670
  • Application notesIs unsuitable for or IP.

    • Target

    • FunctionTranscription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
    • Involvement in diseaseDefects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
    • Sequence similaritiesContains 1 HMG box DNA-binding domain.
    • Post-translational
      modifications      Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
    • Cellular localizationNucleus.

      • Target information above from: UniProt accession P48431 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          ANOP3 antibodycb236 antibodyDelta EF2a antibody
          lcc antibodyMCOPS3 antibodyMGC148683 antibodyMGC2413 antibodyRGD1565646 antibodySex determining region Y box 2 antibodySOX 2 antibodySox2 antibodySOX2_HUMAN antibodySRY (sex determining region Y) box 2 antibodySRY box containing gene 2 antibodySRY related HMG box 2 antibodySRY related HMG box gene 2 antibodySRY-box 2 antibodyTranscription factor SOX 2 antibodyTranscription factor SOX-2 antibodyysb antibody
        see all

      Anti-SOX2 antibody [EPR3131] images

      • Sandwich ELISA - SOX2 antibody [EPR3131] (ab92494)
        Sandwich ELISA - SOX2 antibody [EPR3131] (ab92494)
        Standard Curve for SOX2 (Analyte: SOX2 protein (Human) (ab79950)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [57CT23.3.4] to SOX2 (ab75485) at 0.2µg/ml and Detector Antibody Rabbit monoclonal [EPR3131] to SOX2 (ab92494) at 0.5µg/ml.
      • Western blot - SOX2 antibody [EPR3131] (ab92494)
        Western blot - SOX2 antibody [EPR3131] (ab92494)
        All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/5000 dilution

        Lane 1 : NCCIT cell lysate
        Lane 2 : MCF-7 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size : 34 kDa
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody [EPR3131] (ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody [EPR3131] (ab92494)
        ab92494 at 1/100 dilution staining SOX2 in (1) glioma tissue, (2) Human adult brain tissue & (3) Human fetal brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SOX2 antibody [EPR3131](ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SOX2 antibody [EPR3131](ab92494)
        ab92494 showing negative staining in Seminoma tissue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SOX2 antibody [EPR3131](ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SOX2 antibody [EPR3131](ab92494)
        ab92494 showing positive staining in Breast carcinoma tissue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
        ab92494 showing negative staining in Normal lung tissue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SOX2 antibody [EPR3131](ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SOX2 antibody [EPR3131](ab92494)
        ab92494 showing positive staining in Fetal stomach tissue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
        ab92494 showing positive staining in Fetal lung tissue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)
        ab92494 showing positive staining in Embryonal carcinoma tissue.
      • IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)
        IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)This image is courtesy of an anonymous Abreview.

        IHC-Wholemount image of anti-Sox2 (ab92494) staining on a mouse blastocyst. This blastocyst was stained with anti-Sox2 antibody (pink) at a 1:200 dilution for 48 hours at 4oC. Nuclei stained with DAPI (grey).

        See Abreview

      Protocols

      References for Anti-SOX2 antibody [EPR3131] (ab92494)

      This product has been referenced in:
      • Han X  et al. Silencing SOX2 induced mesenchymal-epithelial transition and its expression predicts liver and lymph node metastasis of CRC patients. PLoS One 7:e41335 (2012). WB, ICC/IF, IHC-P ; Human, Mouse . Read more (PubMed: 22912670) »
      • Lian G  et al. Filamin a regulates neural progenitor proliferation and cortical size through Wee1-dependent Cdk1 phosphorylation. J Neurosci 32:7672-84 (2012). Read more (PubMed: 22649246) »

      See all 4 Publications for this product

      Publishing research using ab92494 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab92494 Submit a Question for ab92494

      Displaying 1 - 7 of 7 results for Abreviews and Q&A

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      Western blot abreview for Anti-SOX2 antibody [EPR3131]

       Excellent
      Abreviews
      Application Western blot
      Sample Human Cell lysate - whole cell (Glioblastoma primary cells)
      Loading amount 30 µg
      Specification Glioblastoma primary cells
      Gel Running Conditions Reduced Denaturing (10%)
      Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
      Read More
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      Verified customer

      Submitted Apr 16 2013

      IHC - Wholemount abreview for Anti-SOX2 antibody [EPR3131]

       Excellent
      Abreviews
      Application IHC - Wholemount
      Sample Mouse Embryo (blastocyst)
      Specification blastocyst
      Read More
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      Username

      Verified customer

      Submitted Feb 06 2013

      The Abcam website says this antibody does not cross-react with mouse, but the Epitomics website says it does. Which is correct?

      Thank you for your inquiry.
      I received verification from Epitomics that they now recommend this antibody for use with mouse due to the fact that it produced a band at the expected molecular weight when tested in WB on a mouse cell line (F9).
      I w...

      Read More

      I am still in the process of searching for high affinity antibodies for SOX2 protein I bought from Abcam (ab95847). Based on what we talked before, you recommended two monoclonal Abs (ab75485, and ab95331) plus a polyclonal (ab75627) against the Sox2 A...

      Read More

      Thank you for contacting us. John is away for a few weeks, my name is Keith.

      While I do not know exactly what you and John spoke of on the phone, from you email I have gathered that you are looking for very high affinity Sox2 antibodies for use...

      Read More

      you very much for the quick reply.
      ab95331 is a monoclonal antibody. I think that I would still need another antibody that would target another epitope of the SOX2 antigen, right?
      The immunogen for this ab95331 is recombinant full length human SOX...

      Read More

      Thank you for your reply.

      As ab95331 is a monoclonal antibody, it will only recognize and bind to one site on the protein, therefore, you would not be able to use it for both the capture and detection antibody. You would have to use it in conjuga...

      Read More


      Thank you very much for your e-mail and the info.
      It seems that ab92494 is not an option for me as the concentration is 10 folds lower than what I need (1 mg/ml).
      As I mentioned to you over the phone yesterday, I already have bought the full ...

      Read More

      Thank you for your reply.

      I think that ab75627 would still be a good option, although you would have to concentrate that antibody, as it comes at 0.25mg/ml. The ab95331, is also another excellent choice, as not only will it work in an ELISA (we h...

      Read More

      What is the sensitivity of ab75627 in ELISA.
      What is the concetrattion of the current lot of ab92494? Need to get it either at 1mg/ml, or 100ug in solid form.

      Thank you for contacting Abcam yesterday.

      I have had to talk to various people in different labs and areas to find out the information that you requested, hence the reason for the delay in the response. I hope it has not been too much of an incon...

      Read More

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