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Immunocytochemistry/ Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
Immunocytochemistry/ Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
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Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
(enlarge)


- SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
- SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
(enlarge)


Product Name 

SOX2 antibody - Embryonic Stem Cell Marker

 

See all SOX2 antibodies (8)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to SOX2 - Embryonic Stem Cell Marker

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SOX2.

(Peptide available as ab15831.)

Reacts with 

Hu, Ms, Rat, Chk, Ql, Zfsh, Dfsh

Predicted to react 
(species key)

Pfsh, Shp, Xl
(Due to sequence homology)

Tested applications 
(see key)

ChIP, ICC/IF, IHC-Fr, IHC-P, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• ChIP

1 Abreview

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

• ICC/IF

4 Abreviews

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• IHC-P

7 Abreviews

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

• IHC-Fr

1 Abreview

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• WB

2 Abreviews

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)


IHC-P
(read Abreview)


ICC/IF
(read Abreview)


ICC/IF
(read Abreview)


ICC/IF
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ChIP: Use at a concentration of 4 µg/ml.
IHC-P: Use at an assay dependent dilution (PMID 19389353).
ICC/IF: Use at a concentration of 3 µg/ml.
IHC-Fr: Use at an assay dependent dilution.
WB: Use at a concentration of 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).

In-house IHC-P testing of this antibody gave inconclusive results. However, we have received several positive reviews from customers using it in that application.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

ICC: human ES cells (H9 line)
WB: mouse or human embryonic stem cell lysate, Mouse Embryonic Germ Cell lysate

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
Neuroscience >> Neurology process >> Neurogenesis
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Neuroscience >> Neurology process >> Neural Signal Transduction
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker

Relevance 

SOX 2 is also known as SRY related HMG BOX gene 2. All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner, resulting in the bending of DNA through large angles. Bending causes the DNA helix to open for some distance, which may affect binding and interactions of other transcription factors. SOX1, SOX2 and SOX3 show the closest homology to SRY. They share maximum homology within the HMG domain and are expressed mainly in the developing nervous system of the mouse. These genes share significant homology outside the HMG box also and are highly conserved throughout their evolution.

 

Alternative names SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

396105    (Chicken)
6657    (Human)
20674    (Mouse)
499593    (Rat)
378723    (Zebrafish)

Omim

    

184429    (Human)

SwissProt

    

P48430    (Chicken)
P48431    (Human)
P48432    (Mouse)
P54231    (Sheep)
O42569    (Xenopus laevis)
Q6P0E1    (Zebrafish)

Unigene

    

518438    (Human)
65396    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SOX2 antibody - Embryonic Stem Cell Marker is on this datasheet. But please do contact us if you would like any reassurance!


See below for SOX2 antibody - Embryonic Stem Cell Marker images, references, products related to ab15830 and other tools.

 

SOX2 antibody - Embryonic Stem Cell Marker images:

Product image  Immunocytochemistry/ Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

Immunocytochemistry/ Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

ICC/IF image of ab15830 stained mouse embryonic stem cells. The cells were 4% formalin fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15830, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

 

Product image  Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

Sox2-specific antibody, ab15830, stained the nucleus of human ES cells (H9 line). Upon differentiation into neurones ab15830, but not Oct4-specific antibody, stained the nuclei (data not shown). Mouse embryonic fibroblasts and ES cells differentiated into early mesoderm were not stained by ab15830.

Dilution used: 5µg/ml.

Ludovic Vallier, University of Cambridge

 

Product image  - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

- SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

Lanes 1 - 2 : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 1 µg/ml

Lane 1: 20 µg Mouse embryonic stem cell lysate
Lane 1: 20 µg Human embryonic stem cell lysate

ab15830 recognised a band of the expected size (34 kDa) in human and mouse embryonic stem cell lysates.

 

Product image  Western blot - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

Western blot - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

All lanes : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 0.3 µg/ml

Lane 1 : Mouse Embryonic Stem Cell lysate
Lane 2 : Mouse Embryonic Germ Cell lysate
Lane 3 : Mouse Embryonic Fibroblast Lysate


Predicted band size : 34 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)

Petra Hajkova, University of Cambridge

 

Product image  Western blot

Western blot

All lanes : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 1 µg/ml

Lane 1 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)
Lane 2 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate (ab27202)
Lane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193)
Lane 4 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) with SOX2 peptide (ab15831) at 1 µg/ml
Lane 5 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate (ab27202) with SOX2 peptide (ab15831) at 1 µg/ml
Lane 6 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193) with SOX2 peptide (ab15831) at 1 µg/ml

Lysates/proteins at 10 µl per lane.


Observed band size : 42 kDa (why is the actual band size different from the predicted?)


A band corresponding to Sox2 was detectable in 46C Sox1-expressing Mouse Neural Progenitor Cell Lysate (ab27205), IOUD2 Oct4-expressing Mouse Embryonic Stem Cell Lysate (ab272020) and F9 Mouse Embryonic Carcinoma Cell Lysate (ab27193). This band was detected in the lysate by Western Blotting using anti-Sox2 antibody ab15830. The Sox2 bands were specifically blocked using the immunising peptide of ab15830. An Oct4 band was also detectable in the 47C lysate by Western Blotting (data not shown). It has not been possible to find a suitable Sox1-specific antibody able to detect Sox1 in 46C by Western Blotting. However, we are confident that 100% of the cells (excluding feeders) that contributed to the lysate were actively expressing Sox1.

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

SOX2 antibody - Embryonic Stem Cell Marker (ab15830) on Formalin/PFA-fixed paraffin-embedded adult zebra fish spinal cord sections. Antigen retrieval step: Heat mediated. 1%BSA as blocking agent for 10 mins at RT°C. ab15830 used at 1/600 for 2 hours.Secondary Antibody: Biotin conjugated goat anti rabbir IgG used at 1/600.

Carl Hobbs, KCL, UK

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

Immunohistochemistical staining (Formaldehyde/PFA-fixed paraffin-embedded sections) for SOX2 antibody - Embryonic Stem Cell Marker (ab15830) on Dogfish/Catshark Tissue sections. Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody used at 1/600 incubated for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti IgG.

Carl Hobbs, CARD, KCL, London, UK

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of SOX2 antibody - Embryonic Stem Cell Marker (ab15830) on Quail Tissue sections (brain stem). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins RT. Primary Antibody ab15830 incubated at 1/600 for 2 hours. Secondary Antibody: Biotin labelled goat anti rabbit IgG. This image shows nuclear expression in the ventricular region of the embryonic quail brain stem.

Carl Hobbs, CARD, KCL, London, UK

 

Product image  Western blot - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

Western blot - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

All lanes : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 1 µg/ml

Lane 1 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)
Lane 2 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate (ab27202)
Lane 3 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 34 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Additional bands at : 97 kDa. We are unsure as to the identity of these extra bands.

 

References for SOX2 antibody - Embryonic Stem Cell Marker (ab15830)

Specific publication references   This product has been used in: (two most recent references)

Moreno-Manzano V et al. FM19G11, a new hypoxia-inducible factor (HIF) modulator, affects stem cell differentiation status. J Biol Chem 285:1333-42 (2010). WB, ICC/IF; Human. PubMed: 19897487

Page RL et al. Induction of stem cell gene expression in adult human fibroblasts without transgenes. Cloning Stem Cells 11:417-26 (2009). WB, ICC/IF; Human. PubMed: 19622035


See all 19 publication references for this product.


If you publish research using ab15830 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to SOX2

 

SOX2 antibody - Embryonic Stem Cell Marker - more information

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Slides

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Loading controls

Lysates

 

Immunizing Peptide for SOX2 antibody - Embryonic Stem Cell Marker

ab15831

  

SOX2 peptide

 

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