|
|
|
|
Immunocytochemistry/ Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
(enlarge)
|
|
Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
(enlarge)
|
|
- SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
(enlarge)
|
|
|
|
|
Product Name
|
SOX2 antibody - Embryonic Stem Cell Marker
|
| |
See all SOX2 antibodies (8)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to SOX2 - Embryonic Stem Cell Marker
|
|
Immunogen
|
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SOX2.
(Peptide available as ab15831.)
|
|
Reacts with
|
Hu, Ms, Rat, Chk, Ql, Zfsh, Dfsh
|
|
Predicted to react
(species key)
|
Pfsh, Shp, Xl
(Due to sequence homology)
|
|
Tested applications
(see key)
|
ChIP, ICC/IF, IHC-Fr, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ChIP: Use at a concentration of 4 µg/ml.
IHC-P: Use at an assay dependent dilution (PMID 19389353).
ICC/IF: Use at a concentration of 3 µg/ml.
IHC-Fr: Use at an assay dependent dilution.
WB: Use at a concentration of 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
In-house IHC-P testing of this antibody gave inconclusive results. However, we have received several positive reviews from customers using it in that application.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
ICC: human ES cells (H9 line)
WB: mouse or human embryonic stem cell lysate, Mouse Embryonic Germ Cell lysate
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
Neuroscience >> Neurology process >> Neurogenesis
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Neuroscience >> Neurology process >> Neural Signal Transduction
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
|
|
Relevance
|
SOX 2 is also known as SRY related HMG BOX gene 2. All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner, resulting in the bending of DNA through large angles. Bending causes the DNA helix to open for some distance, which may affect binding and interactions of other transcription factors. SOX1, SOX2 and SOX3 show the closest homology to SRY. They share maximum homology within the HMG domain and are expressed mainly in the developing nervous system of the mouse. These genes share significant homology outside the HMG box also and are highly conserved throughout their evolution.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.100 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SOX2 antibody - Embryonic Stem Cell Marker is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for SOX2 antibody - Embryonic Stem Cell Marker images, references, products related to ab15830 and other tools.
|
| |
SOX2 antibody - Embryonic Stem Cell Marker images:
|
 Immunocytochemistry/ Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
ICC/IF image of ab15830 stained mouse embryonic stem cells. The cells were 4% formalin fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15830, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
|
| |
Immunofluorescence - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
Sox2-specific antibody, ab15830, stained the nucleus of human ES cells (H9 line). Upon differentiation into neurones ab15830, but not Oct4-specific antibody, stained the nuclei (data not shown). Mouse embryonic fibroblasts and ES cells differentiated into early mesoderm were not stained by ab15830. Dilution used: 5µg/ml. Ludovic Vallier, University of Cambridge
|
| |
- SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
Lanes 1 - 2 : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 1 µg/ml Lane 1: 20 µg Mouse embryonic stem cell lysate
Lane 1: 20 µg Human embryonic stem cell lysate ab15830 recognised a band of the expected size (34 kDa) in human and mouse embryonic stem cell lysates.
|
| |
Western blot - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
All lanes : SOX2 antibody - Embryonic Stem Cell Marker (ab15830) at 0.3 µg/ml
Lane 1 : Mouse Embryonic Stem Cell lysate
Lane 2 : Mouse Embryonic Germ Cell lysate
Lane 3 : Mouse Embryonic Fibroblast Lysate
Predicted band size : 34 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Petra Hajkova, University of Cambridge
|
| |
|
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
SOX2 antibody - Embryonic Stem Cell Marker (ab15830) on Formalin/PFA-fixed paraffin-embedded adult zebra fish spinal cord sections. Antigen retrieval step: Heat mediated. 1%BSA as blocking agent for 10 mins at RT°C. ab15830 used at 1/600 for 2 hours.Secondary Antibody: Biotin conjugated goat anti rabbir IgG used at 1/600. Carl Hobbs, KCL, UK
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
Immunohistochemistical staining (Formaldehyde/PFA-fixed paraffin-embedded sections) for SOX2 antibody - Embryonic Stem Cell Marker (ab15830) on Dogfish/Catshark Tissue sections. Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody used at 1/600 incubated for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti IgG. Carl Hobbs, CARD, KCL, London, UK
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of SOX2 antibody - Embryonic Stem Cell Marker (ab15830) on Quail Tissue sections (brain stem). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins RT. Primary Antibody ab15830 incubated at 1/600 for 2 hours. Secondary Antibody: Biotin labelled goat anti rabbit IgG. This image shows nuclear expression in the ventricular region of the embryonic quail brain stem. Carl Hobbs, CARD, KCL, London, UK
|
| |
|
|
| |
References for SOX2 antibody - Embryonic Stem Cell Marker (ab15830)
|
|
This product has been used in: (two most recent references)
Moreno-Manzano V et al. FM19G11, a new hypoxia-inducible factor (HIF) modulator, affects stem cell differentiation status. J Biol Chem 285:1333-42 (2010). WB, ICC/IF; Human. PubMed: 19897487
Page RL et al. Induction of stem cell gene expression in adult human fibroblasts without transgenes. Cloning Stem Cells 11:417-26 (2009). WB, ICC/IF; Human. PubMed: 19622035
See all 19 publication references for this product.
If you publish research using ab15830 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to SOX2
|
| |
SOX2 antibody - Embryonic Stem Cell Marker - more information
|
Customer reviews (feedback) regarding SOX2 antibody - Embryonic Stem Cell Marker |
Customer FAQs regarding SOX2 antibody - Embryonic Stem Cell Marker |
Protocols for SOX2 antibody - Embryonic Stem Cell Marker |
Price and availability of products related to SOX2 antibody - Embryonic Stem Cell Marker |
| |
SOX2 antibody - Embryonic Stem Cell Marker - related products:
|
|
|
| |
SOX2 antibody - Embryonic Stem Cell Marker - other tools:
|
Contact Abcam with a Technical Enquiry about SOX2 antibody - Embryonic Stem Cell Marker |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
