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ab15831
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab75179? Please let us know so that we can cite the reference in this datasheet
ab75179 has been referenced in 1 publications.
- Cox JL et al. Banf1 is required to maintain the self-renewal of both mouse and human embryonic stem cells. J Cell Sci 124:2654-65 (2011). WB; Human, Mouse. PubMed: 21750191
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - SOX2 antibody - Embryonic Stem Cell Marker (ab75179)
All lanes : Anti-SOX2 antibody - Embryonic Stem Cell Marker (ab75179) at 1 µg/ml
Lane 1 : Hela Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
Lane 5 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 6 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 34 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Additional bands at : 190 kDa. We are unsure as to the identity of these extra bands.
Western blot - SOX2 antibody - Embryonic Stem Cell Marker (ab75179)
All lanes : Anti-SOX2 antibody - Embryonic Stem Cell Marker (ab75179) at 1 µg/ml
Lane 1 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 3 :
Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 34 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
SOX2 antibody - Embryonic Stem Cell Marker for IHC-Fr in Mouse (75179)
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