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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab75179 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - SOX2 antibody - Embryonic Stem Cell Marker (ab75179)
All lanes : Anti-SOX2 antibody - Embryonic Stem Cell Marker (ab75179) at 1 µg/ml
Lane 1 : Hela Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
Lane 5 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 6 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 34 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Additional bands at : 190 kDa. We are unsure as to the identity of these extra bands.
Western blot - SOX2 antibody - Embryonic Stem Cell Marker (ab75179)
All lanes : Anti-SOX2 antibody - Embryonic Stem Cell Marker (ab75179) at 1 µg/ml
Lane 1 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 3 :
Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 34 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
SOX2 antibody - Embryonic Stem Cell Marker for IHC-Fr in Mouse (75179)
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