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Products:Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Overview
Product name
Anti-SOX2 antibody - Embryonic Stem Cell Marker
See all SOX2 products (15) ...
Description
Rabbit polyclonal to SOX2 - Embryonic Stem Cell Marker
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide derived from within residues 1 - 100 SOX2.
(Peptide available as ab15831.)
Positive control
This antibody gave a positive signal in the following whole cell lysates; MEF1, MEL-1, F9, Mouse EG (TMAS), E14Tg2a, Hues7, 46C.
General notes
ab75179 is raised using the same immunogen as ab15830. Batches sold under ab75179 have passed QC testing in Western Blot only. In our hands, this product did not work in ICC/IF. For further information please contact our Customer Services team.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
Neuroscience >> Neurology process >> Neurogenesis
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Neuroscience >> Neurology process >> Neural Signal Transduction
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
Target
Function
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
Involvement in disease
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
Sequence similarities
Contains 1 HMG box DNA-binding domain.
Post-translational
modifications
Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP48431
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
see all
Database links
- Entrez Gene: 6657 Human
- Entrez Gene: 20674 Mouse
- Omim: 184429 Human
- SwissProt: P48431 Human
- SwissProt: P48432 Mouse
- Unigene: 518438 Human
- Unigene: 65396 Mouse
Anti-SOX2 antibody - Embryonic Stem Cell Marker images:
Western blot - SOX2 antibody - Embryonic Stem Cell Marker (ab75179)
All lanes : Anti-SOX2 antibody - Embryonic Stem Cell Marker (ab75179) at 1 µg/ml
Lane 1 : Hela Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
Lane 5 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 6 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 8 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 34 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Additional bands at : 190 kDa. We are unsure as to the identity of these extra bands.
Western blot - SOX2 antibody - Embryonic Stem Cell Marker (ab75179)
All lanes : Anti-SOX2 antibody - Embryonic Stem Cell Marker (ab75179) at 1 µg/ml
Lane 1 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 3 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)
Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 34 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
References for Anti-SOX2 antibody - Embryonic Stem Cell Marker (ab75179)
This product has been referenced in:
- Cox JLet al. Banf1 is required to maintain the self-renewal of both mouse and human embryonic stem cells. J Cell Sci 124:2654-65 (2011). WB; Human, Mouse.Read more (PubMed: 21750191) »
See 1 publication for this product
Publishing research using ab75179? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"