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Western blot - SOX2 antibody (ab59776)
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Immunohistochemistry (PFA perfusion fixed frozen sections) - SOX2 antibody (ab59776)
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Product Name
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SOX2 antibody
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See all SOX2 antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to SOX2
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Immunogen
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Synthetic peptide made from the N-terminal region of human SOX2 protein within residues 1-100.
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Chk
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-Fr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-Fr: 1/125 - 1/250.
WB: 1/2000 - 1/3000. Predicted molecular weight: 40 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human uterus tissue.
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
Neuroscience >> Neurology process >> Neurogenesis
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Neuroscience >> Neurology process >> Neural Signal Transduction
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
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Relevance
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SOX 2 is also known as SRY related HMG BOX gene 2. All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner, resulting in the bending of DNA through large angles. Bending causes the DNA helix to open for some distance, which may affect binding and interactions of other transcription factors. SOX1, SOX2 and SOX3 show the closest homology to SRY. They share maximum homology within the HMG domain and are expressed mainly in the developing nervous system of the mouse. These genes share significant homology outside the HMG box also and are highly conserved throughout their evolution.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 5% Trehalose, PBS
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Form
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Liquid
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Concentration
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Batch dependent within range: 1.81 - 1.81 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SOX2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SOX2 antibody images, references, products related to ab59776 and other tools.
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SOX2 antibody images:
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 Western blot - SOX2 antibody (ab59776)
SOX2 antibody (ab59776) at 0.1 µg/ml + mouse brain lysate
Predicted band size : 40 kDa
Observed band size : 40 kDa
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Immunohistochemistry (PFA perfusion fixed frozen sections) - SOX2 antibody (ab59776)
Ab59776 at 5 µg/ml staining endometrial glands from human uterus; magnification x 20.
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Search PubMed (MEDLINE) for references to SOX2
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SOX2 antibody - more information
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Customer reviews (feedback) regarding SOX2 antibody |
Customer FAQs regarding SOX2 antibody |
Protocols for SOX2 antibody |
Price and availability of products related to SOX2 antibody |
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SOX2 antibody - related products:
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SOX2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about SOX2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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