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Products:Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Overview
Product name
Anti-SOX2 antibody - ChIP Grade
See all SOX2 products (15) ...
Description
Rabbit polyclonal to SOX2 - ChIP Grade
Tested applications
WB, ICC/IF, CHIPseq, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Chicken, Xenopus laevis
Immunogen
Synthetic peptide made from the N-terminal region of human SOX2 protein within residues 1-100.
Positive control
Human uterus tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 5% Trehalose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Developmental Biology >> Reproduction >> Germ cell markers
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
Neuroscience >> Neurology process >> Neurogenesis
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Neuroscience >> Neurology process >> Neural Signal Transduction
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
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Western blot - SOX2 antibody (ab59776)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.2 - 0.5 µg/ml.Predicted molecular weight: 40 kDa.
ICC/IF: 1/200
CHIPseq: Use at an assay dependent dilution. (PubMed: 21211035)
IHC-P
IHC-P: 1/250 - 1/500.IHC-P: 1/250 - 1/500.
Target
Function
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
Involvement in disease
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
Sequence similarities
Contains 1 HMG box DNA-binding domain.
Post-translational
modifications
Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP48431
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
see all
Database links
- Entrez Gene: 396105 Chicken
- Entrez Gene: 6657 Human
- Entrez Gene: 20674 Mouse
- Entrez Gene: 398000 Xenopus laevis
- Omim: 184429 Human
- SwissProt: P48430 Chicken
- SwissProt: P48431 Human
- SwissProt: P48432 Mouse
see all
Anti-SOX2 antibody - ChIP Grade images:
Western blot - SOX2 antibody (ab59776)
Anti-SOX2 antibody - ChIP Grade (ab59776) at 0.1 µg/ml + mouse brain lysate
Predicted band size : 40 kDa
Observed band size : 40 kDa
References for Anti-SOX2 antibody - ChIP Grade (ab59776)
This product has been referenced in:
- Han DWet al. Direct reprogramming of fibroblasts into epiblast stem cells. Nat Cell Biol 13:66-71 (2011). ICC/IF; Mouse.Read more (PubMed: 21131959) »
- O'Reilly LPet al. An unexpected role for the clock protein timeless in developmental apoptosis. PLoS One 6:e17157 (2011). WB; Mouse.Read more (PubMed: 21359199) »
See all 8 publications for this product
Publishing research using ab59776? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"