Overview
- Product nameAnti-SOX2 antibodySee all SOX2 primary antibodies ...
- DescriptionRabbit polyclonal to SOX2
- Tested applicationsIHC-P, ICC/IF, WB, IHC - Wholemount, IHC-Fr more details
- Species reactivityReacts with: Mouse, Rat, Chicken, Human, Zebrafish, Quail, Rainbow Trout, Spotted Catshark (Scyliorhinus canicula)
Predicted to work with: Sheep, Horse, Cow, Pig, Xenopus laevis, Rhesus monkey - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human SOX2.
(Peptide available as ab97974.)
- Positive controlThis antibody gave a positive signal in IOUD2 and HUES7 Whole Cell Lysates.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab97959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: 1/1000 - 1/2000. |
| ICC/IF | ICC/IF: Use a concentration of 1 µg/ml. |
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 34 kDa).Can be blocked with SOX2 peptide (ab97974). |
| IHC - Wholemount | IHC - Wmt: 1/200. |
| IHC-Fr | IHC-Fr: Use at an assay dependent concentration. |
Target
- FunctionTranscription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
- Involvement in diseaseDefects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
- Sequence similaritiesContains 1 HMG box DNA-binding domain.
- Post-translational
modificationsSumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 396105 Chicken
- Entrez Gene: 6657 Human
- Entrez Gene: 20674 Mouse
- Entrez Gene: 499593 Rat
- Entrez Gene: 101110563 Sheep
- Entrez Gene: 398000 Xenopus laevis
- Entrez Gene: 378723 Zebrafish
- Omim: 184429 Human
- SwissProt: P48430 Chicken
- SwissProt: P48431 Human
- SwissProt: P48432 Mouse
- SwissProt: P54231 Sheep
- SwissProt: O42569 Xenopus laevis
- SwissProt: O42569 Xenopus laevis
- SwissProt: Q6P0E1 Zebrafish
- Unigene: 518438 Human
- Unigene: 65396 Mouse
- Unigene: 17369 Rat
- Unigene: 188 Xenopus laevis
- Unigene: 5379 Zebrafish
see all
Target information above from: UniProt accession
P48431
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ANOP3 antibodycb236 antibodyDelta EF2a antibody
- lcc antibodyMCOPS3 antibodyMGC148683 antibodyMGC2413 antibodyRGD1565646 antibodySex determining region Y box 2 antibodySOX 2 antibodySox2 antibodySOX2_HUMAN antibodySRY (sex determining region Y) box 2 antibodySRY box containing gene 2 antibodySRY related HMG box 2 antibodySRY related HMG box gene 2 antibodySRY-box 2 antibodyTranscription factor SOX 2 antibodyTranscription factor SOX-2 antibodyysb antibody
see all
Anti-SOX2 antibody images
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Western blot - Anti-SOX2 antibody (ab97959)All lanes : Anti-SOX2 antibody (ab97959) at 1 µg/ml
Lane 1 : IOUD2 (Mouse embryonic stem cell) Whole Cell Lysate
Lane 2 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 34 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
Additional bands at : 37 kDa,39 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody (ab97959)Carl Hobbs, King`s College London, United KingdomIHC-P image of SOX2 (ab97959) on zebrafish developing retina sections. The sections were fixed in formaldehyde and underwent heat mediated Anigen retrieval using citric acis (pH6). The sections were then blocked in 1% BSA solution for 10 mins at 21°C. The upper section used Abcam's EXPOSE HRP-DAB polyvalent kit (ab80436). The lower section was immunostained using my std stABCpx-DAB method. -
Immunocytochemistry/ Immunofluorescence - SOX2 antibody (ab97959)ICC/IF image of ab97959 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97959, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody (ab97959)Carl Hobbs, King`s College London, United KingdomIHC-P image of SOX2 (ab97959) on E9 Quail developing retina sections. The sections were fixed in formaldehyde and underwent heat mediated Anigen retrieval using citric acis (pH6). The sections were then blocked in 1% BSA solution for 10 mins at 21°C. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody (ab97959)Carl Hobbs, King`s College London, United KingdomIHC-P image of SOX2 (ab97959) on E13 mouse Spinal Cord sections. The sections were fixed in formaldehyde and underwent heat mediated Anigen retrieval using citric acis (pH6). The sections were then blocked in 1% BSA solution for 10 mins at 21°C. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody (ab97959)Carl Hobbs, King`s College London, United KingdomIHC-P image of SOX2 (ab97959) on E7 Chicken brain sections. The sections were fixed in formaldehyde and underwent heat mediated Anigen retrieval using citric acis (pH6). The sections were then blocked in 1% BSA solution for 10 mins at 21°C.The upper section used Abcam's EXPOSE HRP-DAB polyvalent kit (ab80436). The lower section was immunostained using my std stABCpx-DAB method. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody (ab97959)Carl Hobbs, King`s College London, United KingdomIHC-P image of SOX2 (ab97959) on Adult rat brain sections. The sections were fixed in formaldehyde and underwent heat mediated Anigen retrieval using citric acis (pH6). The sections were then blocked in 1% BSA solution for 10 mins at 21°C. Upper image using EXPOSE HRP-DAB polyvalent kit (ab80436) shows strong, clear immunostaining of many nuclei in the subventricular region of the lateral ventricle ( double-red arrowhead) and also of the ependymal cells lining the ventricle ( black arrowheads). Direction of migration indicated by green arrows. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab97959)This image is courtesy of an anonymous Abreviewab97959 staining SOX2 in Spotted Catshark (Scyliorhinus canicula) eye tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/500 in TBS + 1% BSA) for 12 hours at 4°C. An HRP-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody. -
IHC - Wholemount - Anti-SOX2 antibody (ab97959)This image is courtesy of an anonymous abreview.IHC-Wholemount image of anti-Sox2 antibody (ab97959) staining on a mouse embryo. This embryo was stained with anti-Sox2 antibody (green) for 48 hours at 4°C. Nuclei stained with DAPI (grey). -
Western blot - SOX2 protein (Human) (ab79950)Anti-SOX2 antibody (ab97959) at 1/1000 dilution +SOX2 protein (Human) (ab79950) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
References for Anti-SOX2 antibody (ab97959)
This product has been referenced in:
- Miles DC et al. Signaling through the TGF beta-activin receptors ALK4/5/7 regulates testis formation and male germ cell development. PLoS One 8:e54606 (2013). ICC/IF ; Mouse . Read more (PubMed: 23342175) »
- Kelava I et al. Abundant occurrence of basal radial glia in the subventricular zone of embryonic neocortex of a lissencephalic primate, the common marmoset Callithrix jacchus. Cereb Cortex 22:469-81 (2012). IHC-Fr ; Marmoset (common) . Read more (PubMed: 22114084) »
