Overview

  • Product name
    Anti-SOX6 antibody [155C5a]
    See all SOX6 primary antibodies
  • Description
    Mouse monoclonal [155C5a] to SOX6
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human) from the C-terminal end (Accession number: NM_033326)

  • Positive control
    • HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab84880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 93 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • Function
    Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.
  • Tissue specificity
    Expressed in a wide variety of tissues, most abundantly in skeletal muscle.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Post-translational
    modifications
    Sumoylation inhibits the transcriptional activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HSSOX 6 antibody
    • HSSOX6 antibody
    • SOX 6 antibody
    • sox6 antibody
    • SOX6_HUMAN antibody
    • SOXD antibody
    • SRY (sex determining region Y) box 6 antibody
    • SRY box 6 antibody
    • SRY box containing gene 6 antibody
    • Transcription factor SOX 6 antibody
    • Transcription factor Sox-6 antibody
    • Transcription factor SOX6 antibody
    see all

Images

  • Anti-SOX6 antibody [155C5a] (ab84880) + immunizing recombinant protein

    Predicted band size: 93 kDa
    Observed band size: 38 kDa (why is the actual band size different from the predicted?)



    The observed band size may not correspond to the predicted molecular weight as the recombinant immunogen was used for the test lane.
  • Immunostaining analysis in HeLa cells using ab84880. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X 100 in PBS.

References

ab84880 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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