Recombinant Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12755-50] to SOX9 - C-terminal
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SOX9 antibody [EPR12755-50] - C-terminal
See all SOX9 primary antibodies -
Description
Rabbit monoclonal [EPR12755-50] to SOX9 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, SW480 and Caco2 cell lysates; Caco2 and HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12755-50 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/200.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/500.
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WB |
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
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Notes |
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Flow Cyt (Intra)
1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/500. |
WB
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa). |
Target
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Function
Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. -
Involvement in disease
Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6662 Human
- Omim: 608160 Human
- SwissProt: P48436 Human
- Unigene: 647409 Human
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Alternative names
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
see all
Images
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All lanes : Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa -
Immunofluorescence analysis of 4% paraformaldehyde-fixed Caco2 cells, labeling SOX9 with ab184547 at 1/500 dilution (red). Alexa Fluor®555-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
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Immunofluorescence analysis of 4% paraformaldehyde-fixed HepG2 cells, labeling SOX9 with ab184547 at 1/500 dilution (green). Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
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Intracellular flow cytometric analysis of HepG2 cells (2% paraformaldehyde-fixed)labeling SOX9 with ab184547 at 1/200 dilution (red)or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab184547 has been referenced in 2 publications.
- Fabra-Beser J et al. Differential Expression Levels of Sox9 in Early Neocortical Radial Glial Cells Regulate the Decision between Stem Cell Maintenance and Differentiation. J Neurosci 41:6969-6986 (2021). PubMed: 34266896
- Balogh E et al. Hypoxia Triggers Osteochondrogenic Differentiation of Vascular Smooth Muscle Cells in an HIF-1 (Hypoxia-Inducible Factor 1)-Dependent and Reactive Oxygen Species-Dependent Manner. Arterioscler Thromb Vasc Biol 39:1088-1099 (2019). PubMed: 31070451