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ab27845 |
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ab26414 has been referenced in 1 publications.
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Standard Curve for SOX9 ; dilution range 1pg/ml to 1µg/ml using Capture Antibody
All lanes : Anti-SOX9 antibody (ab26414) at 2 µg/ml
Lane 1 : Neuroectoderm (differentiated from human embryonic stem cells) Day 5 - Whole Cell Lysate
Lane 2 : Neuroectoderm (differentiated from human embryonic stem cells) Day 10 - Whole Cell Lysate
Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : Mouse Fetus (14 Day Old) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 56 kDa
Observed band size : 56 kDa
Additional bands at : 100 kDa,90 kDa. We are unsure as to the identity of these extra bands.
Anti-SOX9 antibody (ab26414) at 1 µg/ml + Mouse Fetus (14 Day Old) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 56 kDa
Observed band size : 56 kDa
Exposure time : 90 seconds
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