SOX9 antibody (ab26414)

Product Name 

SOX9 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to SOX9

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SOX9.

(Peptide available as ab27845.)

Reacts with 

Hu, Rat, Pig

Predicted to react 
(species key)

Ms, Cow, Dog, Mmst
(Due to sequence homology)

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

WB: Use at a concentration of 1-2 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Is unsuitable for ICC/IF or IHC-Fr.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Neuroectoderm (HESCS differentiated) Day 5 and Day 10 - Whole Cell Lysate; Caco-2 Whole Cell Lysate, SW480 Whole Cell Lysate, Mouse Fetus (14 day old) Tissue Lysate

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Organogenesis >> Skeletal development >> Bone
Developmental Biology >> Lineage specification >> Ectoderm
Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Stem Cells >> Neural Stem Cells >> Neural Crest Stem Cells
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis

Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Ectoderm
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Neuroscience >> Neurology process >> Neurogenesis
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box

Relevance 

SOX9 is a member of the family of SOX (Sry-type high mobility group box) genes that were first identified on the basis of region with high homology to that of Sry (Sex determining region Y). SOX9 is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. SOX9 is important in neural crest formation, and is involved in regulating subsequent epithelial-mesenchymal transition and migration.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.70 - 0.70 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for SOX9 antibody images, references, products related to ab26414 and other tools.

SOX9 antibody images:

References for SOX9 antibody (ab26414)

  This product has been used in:

Pei M et al. Melatonin enhances cartilage matrix synthesis by porcine articular chondrocytes. J Pineal Res 46:181-7 (2009). WB; Pig. PubMed: 19054299

If you publish research using ab26414 please let us know so that we can cite the reference on this datasheet.

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