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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Overview
Product name
Anti-SOX9 antibody
See all SOX9 products (13) ...
Description
Rabbit polyclonal to SOX9
Tested applications
WB, Sandwich ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Pig
Predicted to work with
Cow, Dog, Marmoset (common)
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SOX9.
(Peptide available as ab27845.)
Positive control
This antibody gave a positive signal in the following lysates: Neuroectoderm (HESCS differentiated) Day 5 and Day 10 - Whole Cell, Caco-2 Whole Cell, SW480 Whole Cell, Mouse Fetus (14 day old) Tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Skeletal development >> Bone
Developmental Biology >> Lineage specification >> Ectoderm
Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Stem Cells >> Neural Stem Cells >> Neural Crest Stem Cells
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Ectoderm
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Neuroscience >> Neurology process >> Neurogenesis
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Applications
Show applications key
Our Abpromise guarantee covers the use of ab26414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1-2 µg/mlDetects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
sELISA: Use a concentration of 0.1 µg/ml Can be paired for Sandwich ELISA with Mouse monoclonal to SOX9 (ab58191). (For sandwich ELISA, use this antibody as Detection at 0.1 µg/ml with Mouse monoclonal to SOX9 (ab58191) as Capture.)
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for ,ICC/IF or IHC-Fr.
Target
Function
Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Involvement in disease
Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
Sequence similarities
Contains 1 HMG box DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP48436
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
see all
Database links
- Entrez Gene: 6662 Human
- Entrez Gene: 20682 Mouse
- Entrez Gene: 140586 Rat
- Omim: 608160 Human
- SwissProt: Q7YRJ7 Dog
- SwissProt: P48436 Human
- SwissProt: Q04887 Mouse
- SwissProt: O18896 Pig
see all
Anti-SOX9 antibody images:
Sandwich ELISA - SOX9 antibody (ab26414)
Standard Curve for SOX9 ; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to SOX9 (ab58191) at 5µg/ml and Detector Antibody Rabbit polyclonal to SOX9 (ab26414) at 0.1µg/ml.
Western blot - SOX9 antibody (ab26414)
All lanes : Anti-SOX9 antibody (ab26414) at 2 µg/ml
Lane 1 : Neuroectoderm (differentiated from human embryonic stem cells) Day 5 - Whole Cell Lysate
Lane 2 : Neuroectoderm (differentiated from human embryonic stem cells) Day 10 - Whole Cell Lysate
Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : Mouse Fetus (14 Day Old) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 56 kDa
Observed band size : 56 kDa
Additional bands at : 100 kDa,90 kDa. We are unsure as to the identity of these extra bands.
Western blot - SOX9 antibody (ab26414)
Anti-SOX9 antibody (ab26414) at 1 µg/ml + Mouse Fetus (14 Day Old) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 56 kDa
Observed band size : 56 kDa
Exposure time : 90 seconds
Protocols
References for Anti-SOX9 antibody (ab26414)
This product has been referenced in:
- Pei Met al. Melatonin enhances cartilage matrix synthesis by porcine articular chondrocytes. J Pineal Res 46:181-7 (2009). WB; Pig.Read more (PubMed: 19054299) »
See 1 publication for this product
Publishing research using ab26414? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"