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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Overview
Product name
Anti-SOX9 antibody
See all SOX9 products (13) ...
Description
Rabbit polyclonal to SOX9
Specificity
Detects endogenous levels of total SOX9 protein.
Tested applications
WB, IHC-P, ELISA, Flow Cytmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic non-phosphopeptide derived from human SOX9 around the phosphorylation site of serine 181 (R-K-SP-V-K).
Positive control
Human brain.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Skeletal development >> Bone
Developmental Biology >> Lineage specification >> Ectoderm
Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Stem Cells >> Neural Stem Cells >> Neural Crest Stem Cells
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Ectoderm
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Neuroscience >> Neurology process >> Neurogenesis
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/5000ELISA: 1/5000
Flow Cyt: Use at an assay dependent concentration. (PubMed: 21818404)
Target
Function
Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Involvement in disease
Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
Sequence similarities
Contains 1 HMG box DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP48436
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
see all
Database links
- Entrez Gene: 6662 Human
- Entrez Gene: 20682 Mouse
- Omim: 608160 Human
- SwissProt: P48436 Human
- SwissProt: Q04887 Mouse
- Unigene: 647409 Human
- Unigene: 286407 Mouse
- Unigene: 481669 Mouse
Anti-SOX9 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - SOX9 antibody (ab59265)
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59265 at a dilution of 1/50-1/100. Left hand image - without immunising peptide; right hand image - with immunising peptide.
Western blot - SOX9 antibody (ab59265)
All lanes : Anti-SOX9 antibody (ab59265) at 1/500 dilution
Lane 1 : extracts from 293 cells, treated
with PBS (60mins)
Lane 2 : extracts from 293 cells, treated
with PBS (60mins) with immunizing non-phosphopeptide
Predicted band size : 56 kDa
Observed band size : 56 kDa
References for Anti-SOX9 antibody (ab59265)
This product has been referenced in:
- Kurth TBet al. Functional mesenchymal stem cell niches in adult mouse knee joint synovium in vivo. Arthritis Rheum 63:1289-300 (2011).Read more (PubMed: 21538315) »
- Handorf AM & Li WJ Fibroblast growth factor-2 primes human mesenchymal stem cells for enhanced chondrogenesis. PLoS One 6:e22887 (2011). WB, Flow Cyt; Human.Read more (PubMed: 21818404) »
See all 3 publications for this product
Publishing research using ab59265? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"