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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Overview
Product name
Anti-SOX9 antibody
See all SOX9 products (13) ...
Description
Rabbit polyclonal to SOX9
Specificity
ab71762 recognizes human SOX9 protein without phosphorylation at S181. It does not recognize SOX9 protein phosphorylated at S181.
Tested applications
EMSA, IHC-P, Dot Blot, WB, IP, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Dog, Human
Predicted to work with
Chicken
Immunogen
Synthetic non-phosphopeptide (Human) surrounding the epitope RKSVK (amino acids 179 - 183) from SOX9.
Positive control
LNCaP prostate Carcinoma whole cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Proclin
Constituents: 10% Glycerol, 1% BSA, Tris buffered saline, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Skeletal development >> Bone
Developmental Biology >> Lineage specification >> Ectoderm
Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Stem Cells >> Neural Stem Cells >> Neural Crest Stem Cells
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Ectoderm
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Neuroscience >> Neurology process >> Neurogenesis
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EMSA: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
Dot: 1/1000
WB: Use a concentration of 0.1 - 1 µg/ml.Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IP: Use a concentration of 2-5 µg/ml
ELISA: Use a concentration of 0.01 - 0.1 µg/ml.
Target
Function
Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Involvement in disease
Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
Sequence similarities
Contains 1 HMG box DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP48436
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
see all
Database links
- Entrez Gene: 374148 Chicken
- Entrez Gene: 6662 Human
- Entrez Gene: 20682 Mouse
- Entrez Gene: 140586 Rat
- Omim: 608160 Human
- SwissProt: P48434 Chicken
- SwissProt: Q7YRJ7 Dog
- SwissProt: P48436 Human
see all
Anti-SOX9 antibody images:
Western blot - SOX9 antibody (ab71762)
All lanes : Anti-SOX9 antibody (ab71762) at 1/500 dilution
Lane 1 : LNCaP prostate
Carcinoma whole cell lysate
Lane 2 : Negative control
Predicted band size : 56 kDa
Observed band size : 56 kDa
Dot Blot - SOX9 antibody (ab71762)
ab71762 at 1/1000 dilution staining 1 µg of the following peptides on nitrocellulose membrane:
A: SOX9 (pS181)
B: SOX9 (Paired S181) (Nonphospho)
C: Non-related phosphospecific PP.
References for Anti-SOX9 antibody (ab71762)
This product has been referenced in:
- Betancur Pet al. Genomic code for Sox10 activation reveals a key regulatory enhancer for cranial neural crest. Proc Natl Acad Sci U S A 107:3570-5 (2010). EMSA.Read more (PubMed: 20139305) »
See 1 publication for this product
Publishing research using ab71762? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"