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Western blot - SOX9 antibody (ab71762)
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Dot Blot - SOX9 antibody (ab71762)
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Product Name
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SOX9 antibody
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See all SOX9 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to SOX9
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Immunogen
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Synthetic non-phosphopeptide (Human) surrounding the epitope RKSVK (amino acids 179 - 183) from SOX9.
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Reacts with
(species key)
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Hu, Ms, Rat, Chk, Cow, Dog
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Specificity
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ab71762 recognizes human SOX9 protein without phosphorylation at S181. It does not recognize SOX9 protein phosphorylated at S181.
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Tested applications
(see key)
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Dot, ELISA, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Dot: 1/1000.
ELISA: Use at a concentration of 0.01 - 0.1 µg/ml.
IHC-P: Use at an assay dependent dilution.
IP: Use at a concentration of 2-5 µg/ml.
WB: Use at a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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LNCaP prostate Carcinoma whole cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Organogenesis >> Skeletal development >> Bone
Developmental Biology >> Lineage specification >> Ectoderm
Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Stem Cells >> Neural Stem Cells >> Neural Crest Stem Cells
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Ectoderm
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Neuroscience >> Neurology process >> Neurogenesis
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
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Relevance
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SOX9 is a member of the family of SOX (Sry-type high mobility group box) genes that were first identified on the basis of region with high homology to that of Sry (Sex determining region Y). SOX9 is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. SOX9 is important in neural crest formation, and is involved in regulating subsequent epithelial-mesenchymal transition and migration.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Proclin
Constituents: 10% Glycerol, 1% BSA, Tris buffered saline, pH 7.4 Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SOX9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SOX9 antibody images, references, products related to ab71762 and other tools.
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SOX9 antibody images:
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 Western blot - SOX9 antibody (ab71762)
All lanes : SOX9 antibody (ab71762) at 1/500 dilution
Lane 1 : LNCaP prostate
Carcinoma whole cell lysate
Lane 2 : Negative control
Predicted band size : 56 kDa
Observed band size : 56 kDa
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Dot Blot - SOX9 antibody (ab71762)
ab71762 at 1/1000 dilution staining 1 µg of the following peptides on nitrocellulose membrane:
A: SOX9 (pS181)
B: SOX9 (Paired S181) (Nonphospho)
C: Non-related phosphospecific PP.
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Search PubMed (MEDLINE) for references to SOX9
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SOX9 antibody - more information
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Customer reviews (feedback) regarding SOX9 antibody |
Customer FAQs regarding SOX9 antibody |
Protocols for SOX9 antibody |
Price and availability of products related to SOX9 antibody |
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SOX9 antibody - related products:
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SOX9 antibody - other tools:
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Contact Abcam with a Technical Enquiry about SOX9 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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