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I can wait. Thanks! Yes, antibody was ordered on July 6 2011 |
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ANSWER: |
I have processed your request to receive ab118892 as a replacement for ab76997. Your new order number is ******, and the antibody will ship out as soon as it comes into stock. |
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Thanks very much. I appreciate your help and promptness! Ab118892 as a replacement would be great. I like the clean WB with 293 extract on the datasheet. |
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ANSWER: |
I would be happy to send you ab118892 as a replacement; it is however on back order for 2 weeks. Would you like to wait or would you like to select a different antibody? Also I believe I have located the original order, but could you just confirm that the antibody was ordered on ********. I look forward to your reply and helping resolve this issue for you. |
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I don't have the catalogue number on hand now (at home), but it is in the second page of the first pdf (the sheet from abcam is scanned onto that page). Let me know if it isn't evident. |
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ANSWER: |
The antibody, ab76997 is recognizing a non-specific band and I was trying to see if we had any information on what that band could be. I have not been able to find any information on the non-specific band and so I am not sure what it could be. As you mentioned in your previous email, as it is recognizing this non-specific band the antibody is unsuitable for you to carry out the assay that you originally purchased it for. Therefore I can send a replacement SOX9 antibody free of charge. Looking through our catalogue, there are a few potential antibodies that would be suitable replacements, these are:ab58191, ab3697. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-SOX9 antibody (ab76997) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 56 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Anti-SOX9 antibody (ab76997) at 5 µg/ml + HepG2 cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 56 kDa
Observed band size : 56 kDa
Immunoperoxidase analysis of SOX9 expression on formalin fixed, paraffin embedded human tonsil tissue using 0.7 µg/ml of ab76997.
Immunofluorescence analysis of SOX9 expression in HepG2 cells, using 10 µg/ml of ab76997.
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