Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Overview
Product name
Anti-SOX9 antibody
See all SOX9 products (13) ...
Description
Mouse monoclonal to SOX9
Tested applications
WB, ELISA, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: EQLSPSHYSE QQQHSPQQIA YSPFNLPHYS PSYPPITRSQ YDYTDHQNSS SYYSHAAGQG TGLYSTFTYM NPAQRPMYTP IADTSGVPSI PQTHSPQHWE QPVYTQLTRP, corresponding to amino acids 400-509 of human SOX9 (NP_000337) with a 26 kDa tag
EQLSPSHYSE QQQHSPQQIA YSPFNLPHYS PSYPPITRSQ YDYTDHQNSS SYYSHAAGQG TGLYSTFTYM NPAQRPMYTP IADTSGVPSI PQTHSPQHWE QPVYTQLTRP
Positive control
Immunogen, HepG2 cell lysate and human tonsil tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Developmental Biology >> Organogenesis >> Skeletal development >> Bone
Developmental Biology >> Lineage specification >> Ectoderm
Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Stem Cells >> Neural Stem Cells >> Neural Crest Stem Cells
Stem Cells >> Mesenchymal Stem Cells >> Chondrogenesis
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Ectoderm
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Neuroscience >> Neurology process >> Neurogenesis
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 56 kDa.
ELISA: Use at an assay dependent dilution. (Detection limit for ab76997 is approximately 30 ng/ml when used as a capture antibody. )
IHC-P: Use a concentration of 0.7 µg/mlPerform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Involvement in disease
Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
Sequence similarities
Contains 1 HMG box DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP48436
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
see all
Database links
- Entrez Gene: 6662 Human
- Omim: 608160 Human
- SwissProt: P48436 Human
- Unigene: 647409 Human
Anti-SOX9 antibody images:
Western blot - SOX9 antibody (ab76997)
Anti-SOX9 antibody (ab76997) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 56 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Western blot - SOX9 antibody (ab76997)
Anti-SOX9 antibody (ab76997) at 5 µg/ml + HepG2 cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 56 kDa
Observed band size : 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX9 antibody (ab76997)
Immunoperoxidase analysis of SOX9 expression on formalin fixed, paraffin embedded human tonsil tissue using 0.7 µg/ml of ab76997.
Immunocytochemistry/ Immunofluorescence - SOX9 antibody (ab76997)
Immunofluorescence analysis of SOX9 expression in HepG2 cells, using 10 µg/ml of ab76997.
References for Anti-SOX9 antibody (ab76997)
This product has been referenced in:
- Kim HMet al. Differential and cooperative actions of Olig1 and Olig2 transcription factors on immature proliferating cells after contusive spinal cord injury. Glia 59:1094-106 (2011).Read more (PubMed: 21538562) »
- Medvedev SPet al. Human induced pluripotent stem cells derived from fetal neural stem cells successfully undergo directed differentiation into cartilage. Stem Cells Dev 20:1099-112 (2011). WB, ICC/IF; Human.Read more (PubMed: 20846027) »
See all 2 publications for this product
Publishing research using ab76997? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"