Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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I would like to test ab13370 for use on mouse and rat samples. |
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DISCOUNT CODE: *** |
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Strong band around 50 kDa and faint band at expected molecular weight with mouse cell line nuclear fraction. |
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Thanks for your call today and for letting us know about the trouble with ab13370. I'm sorry to hear that the results were so poor with this antibody. As we discussed, I am sending a free of charge replacement with ab77441 on the order ***, which should arrive Monday. Please keep me updated about the results with this antibody, and let me know if there is anything else that we can do for you. |
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Dear abcam technical support team: This customer would like to order ab13370 (Anti-SP1 antibody - ChIP Grade), She would likes to order this antibody and test in chip assay with rat sample, would you please help this customer to create a discount code, Thanks for your kindly help. Best regards |
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DISCOUNT CODE: 4XXXXX Expiration date: 28-04-2012 I am very pleased to hear you would like to accept our offer and test ab13370 in ChIP on Rat samples. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ChIP on Rat samples] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. |
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Would like to use in IHC on cryosections (IHC-Fr). |
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ANSWER: |
DISCOUNT CODE: 10*** Expiration date: February 8, 2012
I am very pleased to hear you would like to accept our offer and test ab13370 in IHC-Fr. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab13370 in IHC-Fr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Detection of Human SP1 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate (50 mcg for E; 10 mcg for T) from HEK 293T cells that were mock transfected (E) or transfected with a Sp1 expression construct (T). B. Whole cell lysate (700 mcg) from HEK 293T cells. Antibody: A. Affinity purified rabbit anti-Sp1 antibody (ab13370) used at the indicated concentrations. B. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibodies (lane 2: ab13405, lane 3: ab13370; lane 1: SP-1 antibody, details unknown) using each antibody at 3.75 mcg/mg lysate. Subsequently, ab13370 was used at 0.04 mcg/ml for WB. Detection: Chemiluminescence with an exposure time of 10 seconds (A) or 1 min (B).
ab13370 (2µg/ml) staining SP1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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