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Product Name
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SP1 antibody - ChIP Grade
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See all SP1 antibodies (16)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to SP1 - ChIP Grade
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Immunogen
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Synthetic peptide (Unfortunately the amino acid sequence is considered to be commercially sensitive). The peptide sequence is within the region between aa750 and the C-terminus (aa785) of human SP1 using the numbering given in entry XP_028606.4 (GeneID 666).
BLAST '' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Rat, Cow, Pig, Zfsh
(Due to sequence homology)
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Tested applications
(see key)
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ChIP, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution.
IP: Use at 1-4µg/mg of lysate.
WB: 1/2500 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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Profound changes in patterns of gene expression can result from relatively small changes in the concentrations of sequence specific transcription factors. Synergistic interaction between factors bound to different sites within a transcriptional control region is supported by the work of Courey et al. (1989). Sp1 is a sequence specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. SP1 can interact with G/C rich motifs from serotonin receptor promoter. Kadonaga et al. (1987) cloned the human Sp1 cDNA and showed that it has contiguous zinc finger motifs and requires zinc for sequence specific binding to DNA.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: Tris-citrate/phosphate. pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SP1 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SP1 antibody - ChIP Grade images, references, products related to ab13370 and other tools.
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SP1 antibody - ChIP Grade images:
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 - SP1 antibody (ab13370)
Detection of Human SP1 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate (50 mcg for E; 10 mcg for T) from HEK 293T cells that were mock transfected (E) or transfected with a Sp1 expression construct (T). B. Whole cell lysate (700 mcg) from HEK 293T cells. Antibody: A. Affinity purified rabbit anti-Sp1 antibody (ab13370) used at the indicated concentrations. B. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibodies (lane 2: ab13405, lane 3: ab13370; lane 1: SP-1 antibody, details unknown) using each antibody at 3.75 mcg/mg lysate. Subsequently, ab13370 was used at 0.04 mcg/ml for WB. Detection: Chemiluminescence with an exposure time of 10 seconds (A) or 1 min (B).
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References for SP1 antibody - ChIP Grade (ab13370)
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This product has been used in: (two most recent references)
Turner AM et al. Mobilization-competent Lentiviral Vector-mediated Sustained Transcriptional Modulation of HIV-1 Expression. Mol Ther 17:360-8 (2009). ChIP; Human. PubMed: 19066594
Bierne H et al. Human BAHD1 promotes heterochromatic gene silencing. Proc Natl Acad Sci U S A 106:13826-31 (2009). WB; Human. PubMed: 19666599
See all 4 publication references for this product.
If you publish research using ab13370 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to SP1
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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