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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - SP1 antibody (ab77441)
Anti-SP1 antibody (ab77441) at 5 µg/ml + Recombinant fragment of human SP1 at 0.2 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
Predicted band size : 81 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Western blot - SP1 antibody (ab77441)
Anti-SP1 antibody (ab77441) at 5 µg/ml + IMR-32 cell lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 81 kDa
Observed band size : 81 kDa
Western blot - SP1 antibody (ab77441)
Anti-SP1 antibody (ab77441) at 5 µg/ml + HeLa nuclear cell lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 81 kDa
Observed band size : 81 kDa
Western blot - SP1 antibody (ab77441)
Anti-SP1 antibody (ab77441) at 5 µg/ml + NIH3T3 cell lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 81 kDa
Observed band size : 81 kDa
Immunocytochemistry/ Immunofluorescence - SP1 antibody (ab77441)
ab77441 at 10µg/ml staining SP1 in HeLa cells by Immunofluorescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SP1 antibody (ab77441)
ab77441 staining SP1 in human endometrium by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
Flow Cytometry-SP1 antibody(ab77441)
Overlay histogram showing HeLa cells stained with ab77441 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77441, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
SP1 antibody for Western blot in Rabbit (77441)
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