SP1 (phospho T453) antibody (ab59257)

Product Name 

SP1 (phospho T453) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to SP1 (phospho T453)

Immunogen 

Synthetic phosphopeptide derived from human SP1 around the phosphorylation site of threonine 453 (I-R-T^P-P-T).

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab59257 detects endogenous levels of SP1 only when phosphorylated at
threonine 453.

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/5000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 81 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

IHC-P: Human brain tissue.
WB: A549 cell extracts.

Cellular localization 

Nuclear

Research areas 

Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Relevance 

Profound changes in patterns of gene expression can result from relatively small changes in the concentrations of sequence specific transcription factors. Synergistic interaction between factors bound to different sites within a transcriptional control region is supported by the work of Courey et al. (1989). Sp1 is a sequence specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. SP1 can interact with G/C rich motifs from serotonin receptor promoter. Kadonaga et al. (1987) cloned the human Sp1 cDNA and showed that it has contiguous zinc finger motifs and requires zinc for sequence specific binding to DNA.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab59257 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against
non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

See below for SP1 (phospho T453) antibody images, references, products related to ab59257 and other tools.

SP1 (phospho T453) antibody images:

References for SP1 (phospho T453) antibody (ab59257)

  This product has been used in:

Zhong Z et al. Activated protein C therapy slows ALS-like disease in mice by transcriptionally inhibiting SOD1 in motor neurons and microglia cells. J Clin Invest 119:3437-49 (2009). WB; Mouse. PubMed: 19841542

If you publish research using ab59257 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding SP1 (phospho T453) antibody

Customer FAQs regarding SP1 (phospho T453) antibody

Protocols for SP1 (phospho T453) antibody

Price and availability of products related to SP1 (phospho T453) antibody

SP1 (phospho T453) antibody - related products: