Overview

  • Product name
  • Description
    Rabbit polyclonal to SPANX
  • Specificity
    The immunogen sequence is present within SPANX-A, SPANX-B, SPANX-C, SPANX-D and SPANX-N, but not present within SPANX-E.
  • Tested applications
    Suitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide:

    GDSDPQPAPKKMKTSES

    , corresponding to internal sequence amino acids 31-47 of Human SPANX C

  • Positive control
    • Nuclear extract from VWM105 cells
  • General notes
    Dilute only prior to immediate use.

Properties

Applications

Our Abpromise guarantee covers the use of ab47252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
ICC/IF
  • Application notes
    ELISA: 1/10000 - 1/50000.
    IF (microscopy): Use at an assay dependent dilution.
    WB: 1/500 - 1/3000. Predicted molecular weight: 11 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificity
      Detected in testis, melanoma and bladder carcinoma.
    • Sequence similarities
      Belongs to the SPAN-X family.
    • Developmental stage
      Detected in round and elongating spermatids.
    • Cellular localization
      Cytoplasm. Nucleus. Associated with nuclear craters.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cancer-testis-associated protein CTp11 antibody
      • Cancer/testis antigen 11.3 antibody
      • Cancer/testis-associated protein CTp11 antibody
      • CT11.3 antibody
      • CTP11 antibody
      • Nuclear associated protein SPAN Xc antibody
      • Nuclear-associated protein SPAN-Xc antibody
      • SPANX family member C antibody
      • SPANX family, member C antibody
      • SPANX family, member C1 antibody
      • SPANX-C antibody
      • SPANXC antibody
      • Sperm protein associated with the nucleus on the X chromosome C antibody
      • SPNXC_HUMAN antibody
      see all

    Images

    • All lanes : Anti-SPANX antibody (ab47252) at 1/1000 dilution

      Lane 1 : Nuclear extract from VWM105 cells
      Lane 2 : purified recombinant SPANX-C fusion protein.
      Lane 3 : purified recombinant SPANX-B, protein.
      Lane 4 : purified recombinant SPANX-C, protein.
      Lane 5 : purified recombinant SPANX-N, protein.

      Secondary
      Lanes 1 - 2 : IRDye™800 conjugated Gt-a- Rabbit IgG [H&L] MX (611-132-122)


      Predicted band size : 11 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 kDa,70 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    References

    ab47252 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab47252.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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