Anti-SPANX antibody (ab47252)

Overview

• Product nameAnti-SPANX antibodySee all SPANX primary antibodies ...
• Description
  Rabbit polyclonal to SPANX
• SpecificityThe immunogen sequence is present within SPANX-A, SPANX-B, SPANX-C, SPANX-D and SPANX-N, but not present within SPANX-E.
• Tested applicationsELISA, WB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Chimpanzee, Gorilla
• Immunogen

  Synthetic peptide: GDSDPQPAPKKMKTSES, corresponding to internal sequence amino acids 31-47 of Human SPANX C

  Run BLAST with Run BLAST with
• Positive controlNuclear extract from VWM105 cells
• General notesDilute only prior to immediate use.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Tumor immunology
  • Tumor-associated antigens

  • Cancer
  • Tumor biomarkers
  • Tumor antigens

  • Cell Biology
  • Other Antibodies
  • Other Antibodies

Applications

Target

• Tissue specificityDetected in testis, melanoma and bladder carcinoma.
• Sequence similaritiesBelongs to the SPAN-X family.
• Developmental stageDetected in round and elongating spermatids.
• Cellular localizationCytoplasm. Nucleus. Associated with nuclear craters.

Target information above from: UniProt accession Q9NY87 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 64663 Human
  • Omim: 300330 Human
  • SwissProt: A0FGV4 Human
  • SwissProt: A0FGW6 Human
  • SwissProt: A0FGV1 Human
  • SwissProt: A0FGX0 Human
  • SwissProt: Q32WM2 Human
  • SwissProt: Q9NY87 Human

  • Unigene: 558533 Human

  see all

• Alternative names
  Cancer-testis-associated protein CTp11 antibodyCancer/testis antigen 11.3 antibodyCancer/testis-associated protein CTp11 antibody

  CT11.3 antibodyCTP11 antibodyNuclear associated protein SPAN Xc antibodyNuclear-associated protein SPAN-Xc antibodySPANX family member C antibodySPANX family, member C antibodySPANX family, member C1 antibodySPANX-C antibodySPANXC antibodySperm protein associated with the nucleus on the X chromosome C antibodySPNXC_HUMAN antibody

  see all

Anti-SPANX antibody images

• 

  Western blot - SPANX antibody (ab47252)
  All lanes : Anti-SPANX antibody (ab47252) at 1/1000 dilution
  
  Lane 1 : Nuclear extract from VWM105 cells
  Lane 2 : purified recombinant SPANX-C fusion protein.
  Lane 3 : purified recombinant SPANX-B, protein.
  Lane 4 : purified recombinant SPANX-C, protein.
  Lane 5 : purified recombinant SPANX-N, protein.
  
  Secondary
  Lanes 1 - 2 : IRDye™800 conjugated Gt-a- Rabbit IgG [H&L] MX (611-132-122)
  
  
  Predicted band size : 11 kDa
  Observed band size : 17 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 60 kDa,70 kDa,80 kDa. We are unsure as to the identity of these extra bands.