Overview

  • Product name
  • Description
    Rabbit polyclonal to SPANX
  • Specificity
    Reacts with SPANX-C, SPANX-B and SPANX-N family members.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Full-length recombinant human SPANX-C protein.

  • Positive control
    • Nuclear extract from VWM105 cells, purified recombinant SPANX-B, SPANX-C and SPANX-N proteins.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab51493 was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 11 kDa).
ELISA 1/1000 - 1/4000.
ICC/IF Use at an assay dependent dilution.

Target

  • Tissue specificity
    Detected in testis, melanoma and bladder carcinoma.
  • Sequence similarities
    Belongs to the SPAN-X family.
  • Developmental stage
    Detected in round and elongating spermatids.
  • Cellular localization
    Cytoplasm. Nucleus. Associated with nuclear craters.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer-testis-associated protein CTp11 antibody
    • Cancer/testis antigen 11.3 antibody
    • Cancer/testis-associated protein CTp11 antibody
    • CT11.3 antibody
    • CTP11 antibody
    • Nuclear associated protein SPAN Xc antibody
    • Nuclear-associated protein SPAN-Xc antibody
    • SPANX family member C antibody
    • SPANX family, member C antibody
    • SPANX family, member C1 antibody
    • SPANX-C antibody
    • SPANXC antibody
    • Sperm protein associated with the nucleus on the X chromosome C antibody
    • SPNXC_HUMAN antibody
    see all

Anti-SPANX antibody images

  • All lanes : Anti-SPANX antibody (ab51493) at 1/1000 dilution

    Lane 1 : VWM105 cell nuclear extract
    Lane 2 : purified recombinant SPANX-C fusion protein
    Lane 3 : purified recombinant SPANX-B
    Lane 4 : purified recombinant SPANX-C
    Lane 5 : purified recombinant SPANX-N

    Secondary
    Lane 1 : IRDye™800 conjugated Goat anti Rabbit IgG [H&L]
    Lane 2 : IRDye™800 conjugated Goat anti Rabbit IgG [H&L]


    Predicted band size : 11 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
  • ab51493 at 2.5 ug/ml staining human sperm and spermatids at 60X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

References for Anti-SPANX antibody (ab51493)

ab51493 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your note. This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab51493. For your information, the internal reference note for this credit is ...

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Thank you for getting back to me and for passing some further results. I am very sorry to hear that even after following some tips and recommendations, the non-specific bands will not get weaker on the blot. In case your customer is dissatisfied with t...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this product. After reading through the detailed protocol yo...

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Thank you for your enquiry. I have investigated this with the originator who provided this image. All the samples in lane 2 to 5 of this western blot were recombinant fusion proteins. Consequently, the molecular weights are much higher than expected...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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