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I would like to test ab14174 for use on pig samples. |
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DISCOUNT CODE: *** |
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Hi what is the amino acid sequence used as the antigen for the SPARC antibody?
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Thank you for your enquiry. The immunogen for this antibody is recombinant full length Human protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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Hello, please can you advise me where these SPARC antibodies actually bind to the protein as I am working with tissue sections from a SPARC knockout mouse which has the deletion after exon 5. Knowing whether it's the N or C terminus of the protein will be a great help. ab19528 ab14174 |
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Thank you for your enquiry. Ab19528 has been removed from our catalog. Ab14174 is a polyclonal antibody that was raised to the full length SPARC protein. Therefore, the antibody should recognize epitopes along the entire protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab14174 at a 1/100 dilution staining SPARC in mouse endochondral bone by Immunohistochemistry (frozen sections) incubated for 16 hours at 4°C. PFA fixed. Permeabilized with 0.1% Triton. Blocked using 20% serum for 50 minutes at 19°C. Secondary used at 1/200 polyclonal Donkey anti-rabbit conjugated to Alexa Fluor 555 (red). Counterstain (blue) DAPI.
This image is courtesy of an anonymous abreview.
ab14174 (1/1000) staining SPARC in human substanta nigra using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm and extracellular matrix.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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