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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-SPARC antibody
See all SPARC products (9) ...
Description
Rabbit polyclonal to SPARC
Specificity
There were no cross reactivities obtained with human Osteopontin, and human Bone Sialoprotein.
Tested applications
ELISA, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole Serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Lineage specification >> Endoderm
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Lineage Markers >> Endoderm
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-P: 1/1000Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution.
Target
Function
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Sequence similarities
Belongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Developmental stage
Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
Target information above from: UniProt accessionP09486
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AA517111 antibody
- Basement membrane protein 40 antibody
- Basement-membrane protein 40 antibody
see all
Database links
- Entrez Gene: 6678 Human
- Omim: 182120 Human
- SwissProt: P09486 Human
- Unigene: 111779 Human
- Unigene: 708558 Human
Anti-SPARC antibody images:
Immunohistochemistry (Frozen sections) - SPARC antibody (ab14174)
ab14174 at a 1/100 dilution staining SPARC in mouse endochondral bone by Immunohistochemistry (frozen sections) incubated for 16 hours at 4°C. PFA fixed. Permeabilized with 0.1% Triton. Blocked using 20% serum for 50 minutes at 19°C. Secondary used at 1/200 polyclonal Donkey anti-rabbit conjugated to Alexa Fluor 555 (red). Counterstain (blue) DAPI.
This image is courtesy of an anonymous abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SPARC antibody (ab14174)
ab14174 (1/1000) staining SPARC in human substanta nigra using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm and extracellular matrix.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-SPARC antibody (ab14174)
This product has been referenced in:
- Heller Get al. Genome-wide transcriptional response to 5-aza-2'-deoxycytidine and trichostatin a in multiple myeloma cells. Cancer Res 68:44-54 (2008). IHC-P; Human.Read more (PubMed: 18172295) »
- Synnergren Jet al. Molecular signature of cardiomyocyte clusters derived from human embryonic stem cells. Stem Cells 26:1831-40 (2008). IHC-Fr; Human.Read more (PubMed: 18436862) »
See all 2 publications for this product
Publishing research using ab14174? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"