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Product Name 

SPARC antibody

 

See all SPARC antibodies (8)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to SPARC

Immunogen 

Synthetic peptide: TCDLDNDKYIALDEW, corresponding to amino acids 272-286 of Human SPARC.
BLAST 'TCDLDNDKYIALDEW' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat, Cow
(Due to sequence homology)

Specificity 

There were no cross reactivities obtained with human Osteopontin, and human Bone Sialoprotein.

Tested applications 
(see key)

ELISA


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/3000.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted, extracellular space, extracellular matrix, basement membrane. Note: In or around the basement membrane.

Research areas 

Developmental Biology >> Lineage specification >> Endoderm
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Lineage Markers >> Endoderm
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

Relevance 

SPARC is a secreted protein, acidic and rich in cysteines. It is a matrix associated protein that elicits changes in cell shape, inhibits cell cycle progression, and influences the synthesis of extracellular matrix (ECM).

 

Alternative names SPARC antibody (ab14177)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

386571    (Chicken)
282077    (Cow)
6678    (Human)
20692    (Mouse)
24791    (Rat)

Omim

    

182120    (Human)

SwissProt

    

P36377    (Chicken)
P13213    (Cow)
P09486    (Human)
P07214    (Mouse)
P16975    (Rat)
P36378    (Xenopus laevis)

Unigene

    

111779    (Human)
98989    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: Whole Serum

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SPARC antibody is on this datasheet. But please do contact us if you would like any reassurance!


 

Search PubMed (MEDLINE) for references to SPARC

 

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Other SPARC Antibodies

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SPARC antibody - other tools:

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