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Anti-SPARC antibody (ab14177)

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Overview

Product name

Anti-SPARC antibody
See all SPARC products (9) ...

Description

Rabbit polyclonal to SPARC

Specificity

There were no cross reactivities obtained with human Osteopontin, and human Bone Sialoprotein.

Tested applications

ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chicken, Cow, Xenopus laevis

Immunogen

Synthetic peptide: TCDLDNDKYIALDEW, corresponding to amino acids 272-286 of Human SPARC.

TCDLDNDKYI ALDEW

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole Serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Applications

Show applications key

Our Abpromise guarantee covers the use of ab14177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/3000

    ELISA: 1/3000

Target

Function

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Sequence similarities

Belongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.

Developmental stage

Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.

Cellular localization

Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.

Target information above from: UniProt accessionP09486 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AA517111 antibody
  • Basement membrane protein 40 antibody
  • Basement-membrane protein 40 antibody
  • BM 40 antibody
  • BM-40 antibody
  • BM40 antibody
  • Cysteine rich protein antibody
  • hm:zeh0062 antibody
  • MGC128090 antibody
  • ON antibody
  • Osteonectin antibody
  • Secreted acidic cystein rich glycoprotein antibody
  • Secreted protein acidic and rich in cysteine antibody
  • Secreted protein acidic cysteine rich (osteonectin) antibody
  • Secreted protein acidic cysteine rich antibody
  • SPARC antibody
  • SPRC antibody
  • SPRC_HUMAN antibody
see all

References for Anti-SPARC antibody (ab14177)

ab14177 has not yet been referenced specifically in any publications.

Publishing research using ab14177? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"