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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-SPARC antibody
See all SPARC products (9) ...
Description
Rabbit polyclonal to SPARC
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide derived from the human SPARC protein.
Positive control
Mouse heart lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.08% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purification notes
Purified by SAS precipitation.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Lineage specification >> Endoderm
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Lineage Markers >> Endoderm
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).(PubMed: 19509023)
Target
Function
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Sequence similarities
Belongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Developmental stage
Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
Target information above from: UniProt accessionP09486
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AA517111 antibody
- Basement membrane protein 40 antibody
- Basement-membrane protein 40 antibody
see all
Database links
- Entrez Gene: 6678 Human
- Entrez Gene: 20692 Mouse
- Omim: 182120 Human
- SwissProt: P09486 Human
- SwissProt: P07214 Mouse
- Unigene: 111779 Human
- Unigene: 708558 Human
- Unigene: 291442 Mouse
References for Anti-SPARC antibody (ab55847)
This product has been referenced in:
- Liu Yet al. Apratoxin a reversibly inhibits the secretory pathway by preventing cotranslational translocation. Mol Pharmacol 76:91-104 (2009).Read more (PubMed: 19403701) »
- Kos Ket al. Regulation of the fibrosis and angiogenesis promoter SPARC/osteonectin in human adipose tissue by weight change, leptin, insulin, and glucose. Diabetes 58:1780-8 (2009). WB; Human.Read more (PubMed: 19509023) »
See all 2 publications for this product
Publishing research using ab55847? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"