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Western blot - SPARC antibody (ab55847)
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Product Name
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SPARC antibody
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See all SPARC antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to SPARC
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Immunogen
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Synthetic peptide derived from the human SPARC protein.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1.0 - 5.0 µg/ml. Predicted molecular weight: 35 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse heart lysate
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Cellular localization
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Secreted, extracellular space, extracellular matrix, basement membrane. Note: In or around the basement membrane.
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Research areas
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Developmental Biology >> Lineage specification >> Endoderm
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Lineage Markers >> Endoderm
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Relevance
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SPARC is a secreted protein, acidic and rich in cysteines. It is a matrix associated protein that elicits changes in cell shape, inhibits cell cycle progression, and influences the synthesis of extracellular matrix (ECM).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Storage buffer
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Preservative: 0.08% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified by SAS precipitation.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SPARC antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SPARC antibody images, references, products related to ab55847 and other tools.
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SPARC antibody images:
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References for SPARC antibody (ab55847)
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This product has been used in:
Liu Y et al. Apratoxin a reversibly inhibits the secretory pathway by preventing cotranslational translocation. Mol Pharmacol 76:91-104 (2009). PubMed: 19403701
If you publish research using ab55847 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to SPARC
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SPARC antibody - more information
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Customer reviews (feedback) regarding SPARC antibody |
Customer FAQs regarding SPARC antibody |
Protocols for SPARC antibody |
Price and availability of products related to SPARC antibody |
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SPARC antibody - related products:
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SPARC antibody - other tools:
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Contact Abcam with a Technical Enquiry about SPARC antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Western blot - SPARC antibody (ab55847)