Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-SPARC antibody
See all SPARC products (9) ...
Description
Mouse monoclonal to SPARC
Specificity
Recognises bone SPARC and platelet osteonectin
Tested applications
WB, ELISA, RIAmore details
Cross reactivity
Reacts with
Rat, Cow, Human
Immunogen
Full length native purified Human SPARC
Epitope
First half of the N-terminus of the protein excluding the first 28 amino acids.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, Water
Concentration
Concentration information loading...
Purity
Ion Exchange Chromatography
Purification notes
Salt fractionation followed by gel filtration and ion exchange chromatography
Clonality
Monoclonal
Isotype
IgG1
Research areas
Developmental Biology >> Lineage specification >> Endoderm
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Stem Cells >> Lineage Markers >> Endoderm
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Extracellular Matrix
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
ELISA: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
Target
Function
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Sequence similarities
Belongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Developmental stage
Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
Target information above from: UniProt accessionP09486
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AA517111 antibody
- Basement membrane protein 40 antibody
- Basement-membrane protein 40 antibody
see all
Database links
- Entrez Gene: 282077 Cow
- Entrez Gene: 6678 Human
- Entrez Gene: 24791 Rat
- Omim: 182120 Human
- SwissProt: P13213 Cow
- SwissProt: P09486 Human
- SwissProt: P16975 Rat
- Unigene: 111779 Human
see all
References for Anti-SPARC antibody (ab61383)
ab61383 has not yet been referenced specifically in any publications.
Publishing research using ab61383? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"