Recombinant Anti-SPC24 antibody [EPR11548(B)] (ab169786)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11548(B)] to SPC24
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SPC24 antibody [EPR11548(B)]
See all SPC24 primary antibodies -
Description
Rabbit monoclonal [EPR11548(B)] to SPC24 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293T, MOLT4, 293 and TF1 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11548(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 22 kDa. |
IP
1/10 - 1/100. |
Target
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Function
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. -
Sequence similarities
Belongs to the SPC24 family. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. - Information by UniProt
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Database links
- Entrez Gene: 147841 Human
- Omim: 609394 Human
- SwissProt: Q8NBT2 Human
- Unigene: 381225 Human
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Alternative names
- FLJ90806 antibody
- hSpc24 antibody
- Kinetochore protein Spc24 antibody
see all
Images
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All lanes : Anti-SPC24 antibody [EPR11548(B)] (ab169786) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : MOLT4 cell lysate
Lane 4 : TF1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 22 kDa -
Detection of SPC24 by Western Blot of Immunprecipitate. 293 cell lysate (1) or 1X PBS (2) immunoprecipitated using ab169786 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab169786 has been referenced in 7 publications.
- Uehara DT et al. A missense variant in NUF2, a component of the kinetochore NDC80 complex, causes impaired chromosome segregation and aneuploidy associated with microcephaly and short stature. Hum Genet 140:1047-1060 (2021). PubMed: 33721060
- Wang Y et al. hsa-miR-7-5p suppresses proliferation, migration and promotes apoptosis in hepatocellular carcinoma cell lines by inhibiting SPC24 expression. Biochem Biophys Res Commun 561:80-87 (2021). PubMed: 34020142
- Scott SJ et al. Evidence that polyploidy in esophageal adenocarcinoma originates from mitotic slippage caused by defective chromosome attachments. Cell Death Differ 28:2179-2193 (2021). PubMed: 33649470
- Chen S et al. Increased SPC24 in prostatic diseases and diagnostic value of SPC24 and its interacting partners in prostate cancer. Exp Ther Med 22:923 (2021). PubMed: 34306192
- Petsalaki E et al. Chmp4c is required for stable kinetochore-microtubule attachments. Chromosoma N/A:N/A (2018). PubMed: 29968190
- Schleicher K et al. The Ndc80 complex targets Bod1 to human mitotic kinetochores. Open Biol 7:N/A (2017). WB ; Human . PubMed: 29142109
- Zhou J et al. A potential prognostic biomarker SPC24 promotes tumorigenesis and metastasis in lung cancer. Oncotarget 8:65469-65480 (2017). PubMed: 29029446