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Product Name
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SPEN antibody
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See all SPEN antibodies (2)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to SPEN
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Note on antibody grade |
Fast-Track antibodies
- Immunogen affinity purified
- high ELISA titre values
- released early (to accelerate your research)
- 950 Abpoints to the 1st researcher
who submits conclusive data (positive or negative):
Read the Fast-Track antibody Terms of Use
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Immunogen
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Synthetic peptide: C-DSEGKMDDKKEDHKE, corresponding to Internal sequence amino acids 1503-1517 of Human SPEN
BLAST 'C-DSEGKMDDKKEDHKE' with  or 
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Predicted to react
(species key)
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Hu
(Due to sequence homology)
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Abreviews
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Application notes
(see key)
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Recommended dilutions
This antibody gave a positive result in ELISA against the immunizing peptide. Antibody detection limit dilution 1:4,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear. Associates with chromatin.
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Stem Cells >> Signaling Pathways >> Notch >> Nuclear
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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SPEN is an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. It is a negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. SPEN blocks the differentiation of precursor B-cells into marginal zone B-cells. It probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. SPEN may bind both to DNA and RNA.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SPEN antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to SPEN
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SPEN antibody - more information
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Customer reviews (feedback) regarding SPEN antibody |
Customer FAQs regarding SPEN antibody |
Protocols for SPEN antibody |
Price and availability of products related to SPEN antibody |
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SPEN antibody - related products:
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SPEN antibody - other tools:
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Contact Abcam with a Technical Enquiry about SPEN antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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