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Immunoprecipitation/ Western Blot of SPEN. Lane 1: ab72266 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72266 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 minutes.
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