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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-SPEN antibody
Description
Rabbit polyclonal to SPEN
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from a region between residue 1350 and 1400 of human SPEN, NP_055816.2.
Positive control
Whole cell lysate from Hela cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Immunoprecipitation - SPEN antibody (ab72266)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
- 1 Image
IP
Application notes
IP: Use at 2-5µg/mg of lysate.
WB: Use at a concentration of 1 µg/ml in enriched samples (ie. immunoprecipitates). Predicted molecular weight: 402 kDa. Not suitable for WB in crude samples.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
SPEN is an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. It is a negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. SPEN blocks the differentiation of precursor B-cells into marginal zone B-cells. It probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. SPEN may bind both to DNA and RNA.
Cellular localization
Nuclear. Associates with chromatin.
Alternative names
- MINT antibody
- Msx2 interacting nuclear target (MINT) homolog antibody
- Nuclear receptor transcription cofactor antibody
see all
Database links
- Entrez Gene: 23013 Human
- SwissProt: Q96T58 Human
Anti-SPEN antibody images:
Immunoprecipitation - SPEN antibody (ab72266)
Immunoprecipitation/ Western Blot of SPEN. Lane 1: ab72266 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72266 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 minutes.
References for Anti-SPEN antibody (ab72266)
ab72266 has not yet been referenced specifically in any publications.
Publishing research using ab72266? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"