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Immunoprecipitation - SPEN antibody (ab72266)
Immunoprecipitation - SPEN antibody (ab72266)
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Product Name 

SPEN antibody

 

See all SPEN antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to SPEN

Immunogen 

Synthetic peptide from a region between residue 1350 and 1400 of human SPEN, NP_055816.2.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

IP, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
IP: Use at 2-5µg/mg of lysate.
WB: Use at a concentration of 1 µg/ml in enriched samples (ie. immunoprecipitates). Predicted molecular weight: 402 kDa. Not suitable for WB in crude samples.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Whole cell lysate from Hela cells.

Cellular localization 

Nuclear. Associates with chromatin.

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Stem Cells >> Signaling Pathways >> Notch >> Nuclear
Chromatin and Nuclear Signaling >> Transcription >> Other factors

Relevance 

SPEN is an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. It is a negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. SPEN blocks the differentiation of precursor B-cells into marginal zone B-cells. It probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. SPEN may bind both to DNA and RNA.

 

Alternative names SPEN antibody (ab72266)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

23013    (Human)

SwissProt

    

Q96T58    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SPEN antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for SPEN antibody images, references, products related to ab72266 and other tools.

 

SPEN antibody images:

Product image  Immunoprecipitation - SPEN antibody (ab72266)

Immunoprecipitation - SPEN antibody (ab72266)

Immunoprecipitation/ Western Blot of SPEN.
Lane 1: ab72266 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.

ab72266 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 minutes.

 
 

Search PubMed (MEDLINE) for references to SPEN

 

SPEN antibody - more information

Customer reviews (feedback) regarding SPEN antibody

Customer FAQs regarding SPEN antibody

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Price and availability of products related to SPEN antibody

 

SPEN antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for SPEN antibody

ab29545

  

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

 

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SPEN antibody - other tools:

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