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Immunoprecipitation - SPEN antibody (ab72266)
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Product Name
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SPEN antibody
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See all SPEN antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to SPEN
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Immunogen
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Synthetic peptide from a region between residue 1350 and 1400 of human SPEN, NP_055816.2.
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Reacts with
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Hu
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Tested applications
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 2-5µg/mg of lysate.
WB: Use at a concentration of 1 µg/ml in enriched samples (ie. immunoprecipitates). Predicted molecular weight: 402 kDa. Not suitable for WB in crude samples.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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Whole cell lysate from Hela cells.
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Cellular localization
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Nuclear. Associates with chromatin.
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Stem Cells >> Signaling Pathways >> Notch >> Nuclear
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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SPEN is an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. It is a negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. SPEN blocks the differentiation of precursor B-cells into marginal zone B-cells. It probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. SPEN may bind both to DNA and RNA.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 23013 (Human)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SPEN antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SPEN antibody images, references, products related to ab72266 and other tools.
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SPEN antibody images:
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 Immunoprecipitation - SPEN antibody (ab72266)
Immunoprecipitation/ Western Blot of SPEN.
Lane 1: ab72266 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72266 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 minutes.
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Search PubMed (MEDLINE) for references to SPEN
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SPEN antibody - more information
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Customer reviews (feedback) regarding SPEN antibody |
Customer FAQs regarding SPEN antibody |
Protocols for SPEN antibody |
Price and availability of products related to SPEN antibody |
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SPEN antibody - related products:
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SPEN antibody - other tools:
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Contact Abcam with a Technical Enquiry about SPEN antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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