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Anti-SPEN antibody (ab72266)

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Overview

Product name

Anti-SPEN antibody

Description

Rabbit polyclonal to SPEN

Tested applications

WB, IPmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide from a region between residue 1350 and 1400 of human SPEN, NP_055816.2.

Positive control

Whole cell lysate from Hela cells.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Immunoprecipitation - SPEN antibody (ab72266)Immunoprecipitation - SPEN antibody (ab72266) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab72266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IP: Use at 2-5µg/mg of lysate.
WB: Use at a concentration of 1 µg/ml in enriched samples (ie. immunoprecipitates). Predicted molecular weight: 402 kDa. Not suitable for WB in crude samples.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

SPEN is an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. It is a negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. SPEN blocks the differentiation of precursor B-cells into marginal zone B-cells. It probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. SPEN may bind both to DNA and RNA.

Cellular localization

Nuclear. Associates with chromatin.

Alternative names

  • MINT antibody
  • Msx2 interacting nuclear target (MINT) homolog antibody
  • Nuclear receptor transcription cofactor antibody
  • Nuclear receptor transcription cofactor spen homolog transcriptional regulator antibody
  • RBM15C antibody
  • SHARP antibody
  • SMART/HDAC1 associated repressor protein antibody
  • SPEN homolog antibody
  • Spen homolog transcriptional regulator (Drosophila) antibody
  • Spen homolog transcriptional regulator antibody
see all

Database links

Anti-SPEN antibody images:

  Immunoprecipitation - SPEN antibody (ab72266)

Immunoprecipitation - SPEN antibody (ab72266)

Immunoprecipitation/ Western Blot of SPEN.
Lane 1: ab72266 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.

ab72266 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 minutes.

References for Anti-SPEN antibody (ab72266)

ab72266 has not yet been referenced specifically in any publications.

Publishing research using ab72266? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"