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Anti-SPFH2 antibody (ab36123)

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Overview

Product name

Anti-SPFH2 antibody
See all SPFH2 products (5) ...

Description

Goat polyclonal to SPFH2

Specificity

This antibody is expected to recognise isoform 1 (NP_009106) only.

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Rat

Immunogen

Synthetic peptide: EDEPLETATKEN, corresponding to C terminal amino acids 328-339 of Human SPFH2

EDEPLETATKEN

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Human breast lysates.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline. pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Signal Transduction >> Adapters >> Transmembrane
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Prions

  • Western blot - SPFH2 antibody (ab36123)Western blot - SPFH2 antibody (ab36123) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab36123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 38 kDa).The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pearce et al, J Biol Chem. 2007 Jul 13;282(28):20104-15.; PMID: 17502376).

Target

Function

Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the band 7/mec-2 family.

Cellular localization

Endoplasmic reticulum membrane. Associated with lipid raft-like domains of the endoplasmic reticulum membrane.

Target information above from: UniProt accessionO94905 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • C8orf2 antibody
  • Chromosome 8 open reading frame 2 antibody
  • Endoplasmic reticulum lipid raft associated protein 2 antibody
  • Endoplasmic reticulum lipid raft-associated protein 2 antibody
  • ER lipid raft associated 2 antibody
  • ERLIN 2 antibody
  • Erlin-2 antibody
  • ERLIN2 antibody
  • ERLN2_HUMAN antibody
  • HGNC:1356 antibody
  • MGC87072 antibody
  • NET32 antibody
  • SPFH 2 antibody
  • SPFH domain containing protein 2 antibody
  • SPFH domain family member 2 antibody
  • SPFH domain-containing protein 2 antibody
  • SPFH2 antibody
  • SPG18 antibody
  • Stomatin prohibitin flotillin HflC/K domain containing protein 2 antibody
  • Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2 antibody
see all

Database links

Anti-SPFH2 antibody images:

  Western blot - SPFH2 antibody (ab36123)

Western blot - SPFH2 antibody (ab36123)

Anti-SPFH2 antibody (ab36123) at 1 µg/ml + Human Breast lysate (RIPA buffer, 30µg total protein per lane)

Predicted band size : 38 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)


Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References for Anti-SPFH2 antibody (ab36123)

This product has been referenced in:

  • Sánchez-Quiles V  et al. Prohibitin deficiency blocks proliferation and induces apoptosis in human hepatoma cells: molecular mechanisms and functional implications. Proteomics 10:1609-20 (2010). WB; Human.Read more (PubMed: 20186755) »

See 1 publication for this product

Publishing research using ab36123? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"