Anti-SPG21/Maspardin antibody (ab220229)
Key features and details
- Rabbit polyclonal to SPG21/Maspardin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPG21/Maspardin antibody -
Description
Rabbit polyclonal to SPG21/Maspardin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Cynomolgus monkey, Orangutan -
Immunogen
Recombinant fragment corresponding to Human SPG21/Maspardin aa 200 to the C-terminus.
Database link: Q9NZD8 -
Positive control
- RT-4 and U-251 MG cell lysates; Human liver and tonsil lysates; A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Relevance
Defects in SPG21 are the cause of Mast syndrome, an autosomal recessive hereditary spastic paraplegia with dementia and other CNS abnormalities (SPG21). Present at high frequency among the Old Order Amish. Subtle childhood abnormalities may be present, but the main features develop in early adulthood. The disease is slowly progressive, and cerebellar and extrapyramidal signs are also found in patients with advanced disease. Patients have a thin corpus callosum and white matter abnormalities. The protein encoded by this gene was identified by a two hybrid screen using CD4 as the bait. It binds to the hydrophobic C terminal amino acids of CD4 which are involved in repression of T cell activation. The interaction with CD4 is mediated by the noncatalytic alpha/beta hydrolase fold domain of this protein. It is thus proposed that this gene product modulates the stimulatory activity of CD4. -
Cellular localization
Cytoplasm; cytosol. Membrane; peripheral membrane protein. -
Database links
- Entrez Gene: 404069 Cow
- Entrez Gene: 101866571 Cynomolgus monkey
- Entrez Gene: 51324 Human
- Entrez Gene: 27965 Mouse
- Entrez Gene: 100174603 Orangutan
- NCBI: NP_057714.1 Human
- Omim: 608181 Human
- SwissProt: Q8MJJ1 Cow
see all -
Alternative names
- Acid cluster protein 33 antibody
- ACP33 antibody
- BM019 antibody
see all
Images
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Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized A549 cells labeling SPG21/Maspardin with ab220229 at 4 μg/ml (green).
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All lanes : Anti-SPG21/Maspardin antibody (ab220229) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 35 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab220229 has not yet been referenced specifically in any publications.