Overview
- Product nameAnti-SPHK1 antibodySee all SPHK1 primary antibodies ...
- DescriptionRabbit polyclonal to SPHK1
- Tested applicationsELISA, WB more details
- Species reactivityReacts with: Human
- Immunogen
KLH conjugated synthetic peptide selected from the sequence of human SPHK1 within aa 1-100.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityProtein G purified
- Purification notesThe antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab37980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/1000. |
| WB | WB: 1/100 - 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
Target
- FunctionCatalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
- Tissue specificityWidely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.
- Sequence similaritiesContains 1 DAGKc domain.
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Database links
- Entrez Gene: 8877 Human
- Entrez Gene: 8877 Human
- Entrez Gene: 8877 Human
- Omim: 603730 Human
- SwissProt: Q9NYA1 Human
- SwissProt: Q9NYA1 Human
- SwissProt: Q9NYA1 Human
- Unigene: 68061 Human
Target information above from: UniProt accession
Q9NYA1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- SK 1 antibodySK1 antibodySphingosine Kinase 1 antibody
- Sphingosine Kinase 1 antibodySPHK 1 antibodySPHK antibodySphk1 antibodySPHK1_HUMAN antibodySPK 1 antibodySPK antibodySPK1 antibody
see all
Anti-SPHK1 antibody images
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Western blot - SPHK1 antibody (ab37980)Lane 1 : c-myc tagged SPHK1 antibody
Lane 2 : Anti-SPHK1 antibody (ab37980) at 0.25 mg/ml
Lane 1 : transfected 293 cell lysate
Lane 2 : transfected 293 cell lysate
Predicted band size : 42 kDa
Observed band size : 47 kDa (why is the actual band size different from the predicted?)
Additional bands at : 200 kDa. We are unsure as to the identity of these extra bands.
References for Anti-SPHK1 antibody (ab37980)
This product has been referenced in:
- Usatyuk PV et al. Photolysis of caged sphingosine-1-phosphate induces barrier enhancement and intracellular activation of lung endothelial cell signaling pathways. Am J Physiol Lung Cell Mol Physiol 300:L840-50 (2011). WB ; Human . Read more (PubMed: 21478254) »
