Overview

  • Product nameAnti-SPHK1 antibody
    See all SPHK1 primary antibodies
  • Description
    Rabbit polyclonal to SPHK1
  • Tested applicationsELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the sequence of human SPHK1 within aa 1-100.

  • Positive control
    • IF/ICC: HepG2 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab37980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionCatalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
  • Tissue specificityWidely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.
  • Sequence similaritiesContains 1 DAGKc domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • SK 1 antibody
    • SK1 antibody
    • Sphingosine Kinase 1 antibody
    • Sphingosine Kinase 1 antibody
    • SPHK 1 antibody
    • SPHK antibody
    • Sphk1 antibody
    • SPHK1_HUMAN antibody
    • SPK 1 antibody
    • SPK antibody
    • SPK1 antibody
    see all

Anti-SPHK1 antibody images

  • ICC/IF image of ab37980 stained HepG2 cells. The cells were 4% Formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37980, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Lane 1 : c-myc tagged SPHK1 antibody
    Lane 2 : Anti-SPHK1 antibody (ab37980) at 0.25 mg/ml

    Lane 1 : transfected 293 cell lysate
    Lane 2 : transfected 293 cell lysate


    Predicted band size : 42 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SPHK1 antibody (ab37980)

This product has been referenced in:
  • Usatyuk PV  et al. Photolysis of caged sphingosine-1-phosphate induces barrier enhancement and intracellular activation of lung endothelial cell signaling pathways. Am J Physiol Lung Cell Mol Physiol 300:L840-50 (2011). WB ; Human . Read more (PubMed: 21478254) »

See 1 Publication for this product

Product Wall

Thank you for your email.

Yes, I meant His or GST tag etc.

It is more likely that the band appeared in SPHK2 blot is the specific one. However it can be further testified by
- trying no primary control.
- blocking the antibody...

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Thank you for your email.

It is true that the emails are redirected to us if you reply to mailto:technical@abcam.com. I would advise you to log in into your account and check the CCE thread this is the easiest way for tracking the conversation...

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Thank you for your email.

I am sorry I might have missed the information about LDS buffer as cell lysis buffer - indeed there are companies who sells these for cell lysis.

I have requested specific protocol from laboratory I will send...

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Thank you for contacting us.

Just to confirm I have started working on your inquiry so I will get back to you with more information soon.

There is one comment to make here;

ab37977; This SPHK2 antibody is generated from rabb...

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